No gene-specific primers found for NANOG. Showing all 20 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

NANOG 20 primer pairs found

Human

Show all (including non-specific)

NM_001297698 · chr12

116 2 genes · 3 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' GGTTGCTCCACATTGGAAGG 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 134 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

116 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' GGTTGCTCCACATTGGAAGG 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

116 2 genes · 2 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' TCCCTGGTGGTAGGAAGAGT 3'

Fwd Tm 61.0°C

Rev Tm 61.6°C

GC 55%

Product 140 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

116 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' ATTGGAAGGTTCCCAGTCGG 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 55%

Product 124 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

115 2 genes · 2 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' TCCCTGGTGGTAGGAAGAGT 3'

Fwd Tm 61.0°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' GTCTGGTTGCTCCACATTGG 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 55%

Product 138 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' GGTCTGGTTGCTCCACATTG 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 55%

Product 139 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' GTCTGGTTGCTCCACATTGG 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 55%

Product 139 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' GGTCTGGTTGCTCCACATTG 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 55%

Product 140 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' TGTTCCAGGTCTGGTTGCTC 3'

Fwd Tm 61.0°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' ATTGGAAGGTTCCCAGTCGG 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 55%

Product 123 bp

Spans 2 introns

NCBI BLAST

NM_001297698 · chr12

115 2 genes · 3 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' CAGGTCTGGTTGCTCCACAT 3'

Fwd Tm 61.0°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

114 2 genes · 2 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' TAGGTAGGTGCTGAGGCCTT 3'

Fwd Tm 61.0°C

Rev Tm 62.2°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

114 2 genes · 2 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' TAGGTAGGTGCTGAGGCCTT 3'

Fwd Tm 61.0°C

Rev Tm 62.2°C

GC 55%

Product 108 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

113 2 genes · 2 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' GCATCCCTGGTGGTAGGAAG 3'

Fwd Tm 61.0°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

113 2 genes · 2 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' CATCCCTGGTGGTAGGAAGA 3'

Fwd Tm 61.0°C

Rev Tm 60.3°C

GC 55%

Product 141 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

113 2 genes · 2 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' CATCCCTGGTGGTAGGAAGA 3'

Fwd Tm 61.0°C

Rev Tm 60.3°C

GC 55%

Product 142 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

113 2 genes · 2 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' GCATCCCTGGTGGTAGGAAG 3'

Fwd Tm 61.0°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

111 2 genes · 2 transcripts

FWD 5' GGTGAAGACCTGGTTCCAGA 3'

REV 5' ATCCCTGGTGGTAGGAAGAG 3'

Fwd Tm 61.0°C

Rev Tm 60.1°C

GC 55%

Product 140 bp

Spans 2 introns

NCBI BLAST

NM_024865 · chr12

111 2 genes · 2 transcripts

FWD 5' AGGTGAAGACCTGGTTCCAG 3'

REV 5' ATCCCTGGTGGTAGGAAGAG 3'

Fwd Tm 61.0°C

Rev Tm 60.1°C

GC 55%

Product 141 bp

Spans 2 introns

NCBI BLAST