NELFA 10 primer pairs found

Human

Show all (including non-specific)

NM_005663 · chr4

119 Specific

FWD 5' AGGAACGAGGTGTGAAGCTG 3'

REV 5' GTTCTCCACCACCGTTTCCT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_005663 · chr4

119 Specific

FWD 5' AGGAAGACTCTCGATGCGGA 3'

REV 5' GCAGGCTCATTGTTCAGGGA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_005663 · chr4

119 Specific

FWD 5' CGGAAGGAACGAGGTGTGAA 3'

REV 5' GTTCTCCACCACCGTTTCCT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_005663 · chr4

118 Specific

FWD 5' AGGAAGACTCTCGATGCGGA 3'

REV 5' GCTCATTGTTCAGGGACCCA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_005663 · chr4

118 Specific

FWD 5' CGGAAGGAACGAGGTGTGAA 3'

REV 5' TTCTCCACCACCGTTTCCTC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 140 bp

Spans 2 introns

NM_005663 · chr4

118 Specific

FWD 5' AGGAACGAGGTGTGAAGCTG 3'

REV 5' TTCTCCACCACCGTTTCCTC 3'

Fwd Tm 61.8°C

Rev Tm 61.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_005663 · chr4

118 Specific

FWD 5' GAACGAGGTGTGAAGCTGCT 3'

REV 5' GTTCTCCACCACCGTTTCCT 3'

Fwd Tm 62.3°C

Rev Tm 61.8°C

GC 55%

Product 135 bp

Spans 2 introns

NM_005663 · chr4

117 Specific

FWD 5' AAGACTCTCGATGCGGAGGT 3'

REV 5' GCAGGCTCATTGTTCAGGGA 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 128 bp

Spans 2 introns

NM_005663 · chr4

117 Specific

FWD 5' AGGAAGACTCTCGATGCGGA 3'

REV 5' AGGCTCATTGTTCAGGGACC 3'

Fwd Tm 62.3°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 2 introns

NM_005663 · chr4

117 Specific

FWD 5' AAGACTCTCGATGCGGAGGT 3'

REV 5' GCTCATTGTTCAGGGACCCA 3'

Fwd Tm 62.6°C

Rev Tm 62.0°C

GC 55%

Product 124 bp

Spans 2 introns