NM_001102654 · chr12

110 Specific

FWD 5' CGCGTCCACCTTTCTCTTCA 3'

REV 5' GTTACCTTGGATGCCACGGA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' CCACACGGATGCCATGGTTA 3'

REV 5' TGTTACCTTGGATGCCACGG 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 110 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' ATGTCGACGTCCCTGGAAAC 3'

REV 5' GTTACCTTGGATGCCACGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' CATGTCGACGTCCCTGGAAA 3'

REV 5' TGGATGCCACGGAGATAAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 125 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' ATGTCGACGTCCCTGGAAAC 3'

REV 5' TGGATGCCACGGAGATAAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 124 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' CATGTCGACGTCCCTGGAAA 3'

REV 5' GTTACCTTGGATGCCACGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 132 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' CTCGCGTCCACCTTTCTCTT 3'

REV 5' TGGATGCCACGGAGATAAGC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' TCGCGTCCACCTTTCTCTTC 3'

REV 5' TGGATGCCACGGAGATAAGC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 144 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' CGCGTCCACCTTTCTCTTCA 3'

REV 5' TGGATGCCACGGAGATAAGC 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 143 bp

Spans 1 intron

NCBI BLAST

NM_001102654 · chr12

109 Specific

FWD 5' TCGCGTCCACCTTTCTCTTC 3'

REV 5' ATGCCACGGAGATAAGCGAG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 141 bp

Spans 1 intron

NCBI BLAST

NM_002527 · chr12

80 Single Exon 1 gene · 2 transcripts

FWD 5' AACGGTACGCGGAGCATAAG 3'

REV 5' TGTCGATGGCCGATGACTTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 93 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon 1 gene · 2 transcripts

FWD 5' ACAAGTCATCGGCCATCGAC 3'

REV 5' CTGGCTTCCTTACATCGCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 115 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon Specific

FWD 5' TGCCAGAGCCTGCTCTTAAC 3'

REV 5' GTTACCTTGGATGCCACGGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 117 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon Specific

FWD 5' GCCAGAGCCTGCTCTTAACA 3'

REV 5' GTTACCTTGGATGCCACGGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon 1 gene · 2 transcripts

FWD 5' CAAGTCATCGGCCATCGACA 3'

REV 5' CTGGCTTCCTTACATCGCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 114 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon 1 gene · 2 transcripts

FWD 5' GAAACGGTACGCGGAGCATA 3'

REV 5' TGTCGATGGCCGATGACTTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 95 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

80 Single Exon 1 gene · 2 transcripts

FWD 5' AACGGTACGCGGAGCATAAG 3'

REV 5' GTCGATGGCCGATGACTTGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 92 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

79 Single Exon 1 gene · 2 transcripts

FWD 5' GAAACGGTACGCGGAGCATA 3'

REV 5' GTCGATGGCCGATGACTTGT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 94 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

79 Single Exon 1 gene · 2 transcripts

FWD 5' TCATCGGCCATCGACATTCG 3'

REV 5' CTGGCTTCCTTACATCGCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 110 bp

Single-exon gene (no intron)

NCBI BLAST

NM_002527 · chr12

79 Single Exon 1 gene · 2 transcripts

FWD 5' AACGGTACGCGGAGCATAAG 3'

REV 5' CGAATGTCGATGGCCGATGA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 97 bp

Single-exon gene (no intron)

NCBI BLAST

NTF3 20 primer pairs found