Nefl 10 primer pairs found

Human

Show all (including non-specific)

NM_006158 · chr8

109 Specific

FWD 5' ATCAGCGCTATGCAGGACAC 3'

REV 5' CTGGTTATGCTTCCCACGCT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 200 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' CATCAGCGCTATGCAGGACA 3'

REV 5' ACGCTGGTGAAACTGAGTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 186 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' ACATCAGCGCTATGCAGGAC 3'

REV 5' ACGCTGGTGAAACTGAGTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 187 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' ATCAGCGCTATGCAGGACAC 3'

REV 5' ACGCTGGTGAAACTGAGTCG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 185 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' CGCTATGCAGGACACGATCA 3'

REV 5' AGCCACTGGTTATGCTTCCC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 200 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' ACATCAGCGCTATGCAGGAC 3'

REV 5' CCACGCTGGTGAAACTGAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 189 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' ATCAGCGCTATGCAGGACAC 3'

REV 5' CCACGCTGGTGAAACTGAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 187 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' GACATCAGCGCTATGCAGGA 3'

REV 5' ACGCTGGTGAAACTGAGTCG 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 188 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' CATCAGCGCTATGCAGGACA 3'

REV 5' CCACGCTGGTGAAACTGAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 188 bp

Spans 2 introns

NM_006158 · chr8

109 Specific

FWD 5' ACATCAGCGCTATGCAGGAC 3'

REV 5' TCTCCTCGCCTTCCAAGAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 165 bp

Spans 2 introns