Nipa1 20 primer pairs found

Human

Show all (including non-specific)

NM_144599 · chr15

118 Specific

FWD 5' ACAGAAGAAGGGCATCGTGC 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_144599 · chr15

118 Specific

FWD 5' AGCGGCGAGGTACTTCCTAT 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 97 bp

Spans 2 introns

NM_144599 · chr15

117 Specific

FWD 5' AAGCGGCGAGGTACTTCCTA 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 98 bp

Spans 2 introns

NM_144599 · chr15

117 Specific

FWD 5' CGTGCTACAGAAGAAGGGCA 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 129 bp

Spans 2 introns

NM_144599 · chr15

116 Specific

FWD 5' ACAGAAGAAGGGCATCGTGC 3'

REV 5' TTCCAATCTGGCCAACAGCC 3'

Fwd Tm 62.4°C

Rev Tm 62.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_144599 · chr15

116 Specific

FWD 5' GTCCACGTTCGTGCTACAGA 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 61.6°C

Rev Tm 62.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_144599 · chr15

116 Specific

FWD 5' TCGTGCTACAGAAGAAGGGC 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 61.5°C

Rev Tm 62.4°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001142275 · chr15

115 Specific

FWD 5' GCGCTCCGGTACTTCCTATT 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 61.4°C

Rev Tm 62.4°C

GC 55%

Product 96 bp

Spans 2 introns

NM_144599 · chr15

115 Specific

FWD 5' GCGGCGAGGTACTTCCTATT 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 61.4°C

Rev Tm 62.4°C

GC 55%

Product 96 bp

Spans 2 introns

NM_144599 · chr15

115 Specific

FWD 5' CGTGCTACAGAAGAAGGGCA 3'

REV 5' TTCCAATCTGGCCAACAGCC 3'

Fwd Tm 61.8°C

Rev Tm 62.8°C

GC 55%

Product 110 bp

Spans 2 introns

NM_144599 · chr15

115 Specific

FWD 5' GGTCCACGTTCGTGCTACAG 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 62.7°C

Rev Tm 62.4°C

GC 60%

Product 139 bp

Spans 2 introns

NM_001142275 · chr15

114 Specific

FWD 5' GCGCTCCGGTACTTCCTATT 3'

REV 5' GACCCGAACGGTACTCCAAG 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001142275 · chr15

110 Specific

FWD 5' CGCGCTCCGGTACTTCCTAT 3'

REV 5' GCCGTGTAAGCCAGGAAGTT 3'

Fwd Tm 63.6°C

Rev Tm 62.4°C

GC 60%

Product 97 bp

Spans 2 introns

NM_001142275 · chr15

110 1 gene · 2 transcripts

FWD 5' GGCTCCGTCGTGCTGATTAT 3'

REV 5' TCCAGAAGATGAGCAGCAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001142275 · chr15

110 1 gene · 2 transcripts

FWD 5' TGTGACAACTCAGGCTGAGC 3'

REV 5' TCCAGAAGATGAGCAGCAGC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 101 bp

Spans 1 intron

NM_001142275 · chr15

109 1 gene · 2 transcripts

FWD 5' GGCTCCGTCGTGCTGATTAT 3'

REV 5' CGCGATCCAGAAGATGAGCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001142275 · chr15

109 1 gene · 2 transcripts

FWD 5' TGTGACAACTCAGGCTGAGC 3'

REV 5' CGCGATCCAGAAGATGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001142275 · chr15

109 1 gene · 2 transcripts

FWD 5' TGTGACAACTCAGGCTGAGC 3'

REV 5' CCAGAAGATGAGCAGCAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001142275 · chr15

109 1 gene · 2 transcripts

FWD 5' GTGACAACTCAGGCTGAGCT 3'

REV 5' CGCGATCCAGAAGATGAGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001142275 · chr15

109 1 gene · 2 transcripts

FWD 5' TGACAACTCAGGCTGAGCTG 3'

REV 5' CGCGATCCAGAAGATGAGCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 104 bp

Spans 1 intron