Nphs1 10 primer pairs found

Human

Show all (including non-specific)

NM_004646 · chr19

120 Specific

FWD 5' AGAGAAGACAGAGGCAGGGT 3'

REV 5' TCTGCCTCTGTTGTGCTGAC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 2 introns

NM_004646 · chr19

118 Specific

FWD 5' GTCTTGCTGAGGGCATCTCA 3'

REV 5' ATACGGCTCTGCCTCTGTTG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 140 bp

Spans 3 introns

NM_004646 · chr19

118 Specific

FWD 5' GTCTTGCTGAGGGCATCTCA 3'

REV 5' CTCTGCCTCTGTTGTGCTGA 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 3 introns

NM_004646 · chr19

118 Specific

FWD 5' AGAGAAGACAGAGGCAGGGT 3'

REV 5' CTCTGCCTCTGTTGTGCTGA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 115 bp

Spans 2 introns

NM_004646 · chr19

118 Specific

FWD 5' GTCTTGCTGAGGGCATCTCA 3'

REV 5' TCTGCCTCTGTTGTGCTGAC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 133 bp

Spans 3 introns

NM_004646 · chr19

118 Specific

FWD 5' TCCTCTCCAATGCCTCCTGT 3'

REV 5' TTCGTTCCTGACTCGGTCCT 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 119 bp

Spans 2 introns

NM_004646 · chr19

118 Specific

FWD 5' TCCTCTCCAATGCCTCCTGT 3'

REV 5' TGTCCACTGGCTCTCCTCAT 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 140 bp

Spans 2 introns

NM_004646 · chr19

118 Specific

FWD 5' AGAGAAGACAGAGGCAGGGT 3'

REV 5' TCCCTCAGGGAGCGGTAATA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 2 introns

NM_004646 · chr19

118 Specific

FWD 5' AGAGAAGACAGAGGCAGGGT 3'

REV 5' ATACGGCTCTGCCTCTGTTG 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 121 bp

Spans 2 introns

NM_004646 · chr19

117 Specific

FWD 5' AGAGAAGACAGAGGCAGGGT 3'

REV 5' CGGTAATACGGCTCTGCCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 2 introns