Nphs2 20 primer pairs found

Human

Show all (including non-specific)

NM_014625 · chr1

120 Specific

FWD 5' GGAAGAGCATCGCCCAAGAT 3'

REV 5' ACAGCCAGTGAGTGCTGAAG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_014625 · chr1

120 Specific

FWD 5' CGCCCAAGATGCAAAGGTTG 3'

REV 5' ACAGCCAGTGAGTGCTGAAG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 117 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CGCCCAAGATGCAAAGGTTG 3'

REV 5' CTTGTCTTTGCGCTTCAGCC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CGCCCAAGATGCAAAGGTTG 3'

REV 5' GGCTTGTCTTTGCGCTTCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CCCAAGATGCAAAGGTTGCC 3'

REV 5' CTTGTCTTTGCGCTTCAGCC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CCCAAGATGCAAAGGTTGCC 3'

REV 5' GGCTTGTCTTTGCGCTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' GGAAGAGCATCGCCCAAGAT 3'

REV 5' CTTGTCTTTGCGCTTCAGCC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CGCCCAAGATGCAAAGGTTG 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 95 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' CCCAAGATGCAAAGGTTGCC 3'

REV 5' ACAGCCAGTGAGTGCTGAAG 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 115 bp

Spans 2 introns

NM_014625 · chr1

119 Specific

FWD 5' GCCCAAGATGCAAAGGTTGC 3'

REV 5' CACAGCCAGTGAGTGCTGAA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001297575 · chr1

117 1 gene · 2 transcripts

FWD 5' TTGCCCTGCCTGGATACCTA 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001297575 · chr1

114 1 gene · 2 transcripts

FWD 5' CCTGCCTGGATACCTACCAC 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 60%

Product 144 bp

Spans 2 introns

NM_001297575 · chr1

114 1 gene · 2 transcripts

FWD 5' CCCTGCCTGGATACCTACCA 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 62.8°C

Rev Tm 62.2°C

GC 60%

Product 145 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' GCTTCCTGGAAGAGCCAAAG 3'

REV 5' CAGGTCACTGAATCCAAGGC 3'

Fwd Tm 60.6°C

Rev Tm 60.4°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' TTTGCCCTGCCTGGATACCT 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 63.4°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' CCTGCCTGGATACCTACCAC 3'

REV 5' AGCTGGCAACCTCACATCTT 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 60%

Product 143 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' GACTGGGACATCTGCTTCCT 3'

REV 5' AGGTCACTGAATCCAAGGCA 3'

Fwd Tm 61.1°C

Rev Tm 60.9°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' GACTGGGACATCTGCTTCCT 3'

REV 5' CAGGTCACTGAATCCAAGGC 3'

Fwd Tm 61.1°C

Rev Tm 60.4°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001297575 · chr1

113 1 gene · 2 transcripts

FWD 5' ACCACAAGGTTGACCTTCGT 3'

REV 5' CAGCTGGCAACCTCACATCT 3'

Fwd Tm 61.2°C

Rev Tm 62.2°C

GC 50%

Product 129 bp

Spans 2 introns

NM_001297575 · chr1

112 1 gene · 2 transcripts

FWD 5' ACTGGGACATCTGCTTCCTG 3'

REV 5' AGGTCACTGAATCCAAGGCA 3'

Fwd Tm 61.4°C

Rev Tm 60.9°C

GC 55%

Product 143 bp

Spans 2 introns