PCLO 20 primer pairs found

Human

Show all (including non-specific)

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.3°C

Rev Tm 60.2°C

GC 50%

Product 142 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.3°C

Rev Tm 60.2°C

GC 50%

Product 142 bp

Spans 2 introns

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' TCCCAAACTGTCACCTCCAG 3'

Fwd Tm 60.3°C

Rev Tm 61.3°C

GC 50%

Product 144 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' TCCCAAACTGTCACCTCCAG 3'

Fwd Tm 60.3°C

Rev Tm 61.3°C

GC 50%

Product 144 bp

Spans 2 introns

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' CCAGAATGCAAGTGCTGAGT 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.1°C

Rev Tm 60.2°C

GC 50%

Product 139 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' CCAGAATGCAAGTGCTGAGT 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.1°C

Rev Tm 60.2°C

GC 50%

Product 139 bp

Spans 2 introns

NM_033026 · chr7

110 Specific

FWD 5' CGTGCAACAACTCCGCATTC 3'

REV 5' TGCTTCCTTCACTGTCCACG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 125 bp

Spans 1 intron

NM_033026 · chr7

110 Specific

FWD 5' CGTGCAACAACTCCGCATTC 3'

REV 5' GCTGCTTCCTTCACTGTCCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_033026 · chr7

110 Specific

FWD 5' CGTGCAACAACTCCGCATTC 3'

REV 5' TGCTGCTTCCTTCACTGTCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 128 bp

Spans 1 intron

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' CCCAAACTGTCACCTCCAGT 3'

Fwd Tm 60.3°C

Rev Tm 61.6°C

GC 50%

Product 143 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' TGTCCAGAATGCAAGTGCTG 3'

REV 5' CCCAAACTGTCACCTCCAGT 3'

Fwd Tm 60.3°C

Rev Tm 61.6°C

GC 50%

Product 143 bp

Spans 2 introns

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' GTCCAGAATGCAAGTGCTGA 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.1°C

Rev Tm 60.2°C

GC 50%

Product 141 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' GTCCAGAATGCAAGTGCTGA 3'

REV 5' CCAAACTGTCACCTCCAGTG 3'

Fwd Tm 60.1°C

Rev Tm 60.2°C

GC 50%

Product 141 bp

Spans 2 introns

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' CATTCCAGTCAGAGCAGCCA 3'

REV 5' TCTCAATGGTGGGAACCTGC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 109 bp

Spans 1 intron

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' CATTCCAGTCAGAGCAGCCA 3'

REV 5' TCTCAATGGTGGGAACCTGC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 109 bp

Spans 1 intron

NM_033026 · chr7

110 1 gene · 2 transcripts

FWD 5' CAGCAAGCCTACCGATGGAA 3'

REV 5' ACCTCTCCCTGGAAGAAGGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 184 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' CAGCAAGCCTACCGATGGAA 3'

REV 5' ACCTCTCCCTGGAAGAAGGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 184 bp

Spans 2 introns

NM_014510 · chr7

110 1 gene · 2 transcripts

FWD 5' AGCAAGCCTACCGATGGAAC 3'

REV 5' ACCTCTCCCTGGAAGAAGGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 183 bp

Spans 2 introns

NM_014510 · chr7

109 1 gene · 2 transcripts

FWD 5' TCACGGACTCGAGGCTATGA 3'

REV 5' CTGTTTCCTCACGACGCAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 103 bp

Spans 1 intron

NM_014510 · chr7

109 1 gene · 2 transcripts

FWD 5' TCACGGACTCGAGGCTATGA 3'

REV 5' TCTGTTTCCTCACGACGCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 104 bp

Spans 1 intron