PDE6A 10 primer pairs found

Human

Show all (including non-specific)

NM_000440 · chr5

119 Specific

FWD 5' CACATTCCACAAGAGGCCCT 3'

REV 5' AGTAGCGCTTCAGCTTTCCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' ACATTCCACAAGAGGCCCTG 3'

REV 5' AGTAGCGCTTCAGCTTTCCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' TCACATTCCACAAGAGGCCC 3'

REV 5' AGTAGCGCTTCAGCTTTCCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' ATGATGCTGGAGCAGACACG 3'

REV 5' TCCCAGAATTCAGCAGCCAC 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' TCCACCTTACTCTGGTCCCA 3'

REV 5' AAAGCCCAATGGTCAGGAGG 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' AAATCCCAGAACCCACTGGC 3'

REV 5' CATGCTCATGCTGTCGACGA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_000440 · chr5

119 Specific

FWD 5' TCCACCTTACTCTGGTCCCA 3'

REV 5' AGCTGGGAGACCGCTTACTA 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_000440 · chr5

118 Specific

FWD 5' CACGGTGCTGCAACAGAATC 3'

REV 5' CCTCGTGGAAACGGGAGAAT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 131 bp

Spans 2 introns

NM_000440 · chr5

118 Specific

FWD 5' AGGACTCCGGATGGAAGAGA 3'

REV 5' CATAAGCTGGGAGACCGCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 2 introns

NM_000440 · chr5

118 Specific

FWD 5' ACCTTACTCTGGTCCCAGGA 3'

REV 5' CATAAGCTGGGAGACCGCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 2 introns