PFN1 20 primer pairs found

Human

Show all (including non-specific)

NM_005022 · chr17

108 Specific

FWD 5' AATGTTCGGTGATCCGGGAC 3'

REV 5' TCTTTGCCCATCAGCAGGAC 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001375991 · chr17

108 1 gene · 2 transcripts

FWD 5' ACATCGACAACCTCATGGCG 3'

REV 5' TCTTTGCCAACCAGGACACC 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001375991 · chr17

108 1 gene · 2 transcripts

FWD 5' ACATCGACAACCTCATGGCG 3'

REV 5' GGTCTTTGCCAACCAGGACA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 1 intron

NM_005022 · chr17

108 1 gene · 2 transcripts

FWD 5' ACATCGACAACCTCATGGCG 3'

REV 5' TCTTTGCCAACCAGGACACC 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 145 bp

Spans 1 intron

NM_005022 · chr17

108 1 gene · 2 transcripts

FWD 5' ACATCGACAACCTCATGGCG 3'

REV 5' GGTCTTTGCCAACCAGGACA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 1 intron

NM_005022 · chr17

108 Specific

FWD 5' AATGTTCGGTGATCCGGGAC 3'

REV 5' CTTTGCCCATCAGCAGGACT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001375991 · chr17

108 1 gene · 2 transcripts

FWD 5' TCAACATCACGCCAGCTGAG 3'

REV 5' TCACCGAACATTTCTGGCCC 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 99 bp

Spans 1 intron

NM_005022 · chr17

108 1 gene · 2 transcripts

FWD 5' TCAACATCACGCCAGCTGAG 3'

REV 5' TCACCGAACATTTCTGGCCC 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 99 bp

Spans 1 intron

NM_005022 · chr17

108 Specific

FWD 5' GGGCCAGAAATGTTCGGTGA 3'

REV 5' CTTTGCCCATCAGCAGGACT 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 149 bp

Spans 1 intron

NM_005022 · chr17

108 Specific

FWD 5' GGGCCAGAAATGTTCGGTGA 3'

REV 5' TCTTTGCCCATCAGCAGGAC 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001375991 · chr17

108 1 gene · 2 transcripts

FWD 5' TCAACATCACGCCAGCTGAG 3'

REV 5' GTCCCGGATCACCGAACATT 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_005022 · chr17

108 1 gene · 2 transcripts

FWD 5' TCAACATCACGCCAGCTGAG 3'

REV 5' GTCCCGGATCACCGAACATT 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_001375991 · chr17

108 1 gene · 2 transcripts

FWD 5' GTCAACATCACGCCAGCTGA 3'

REV 5' TCACCGAACATTTCTGGCCC 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 100 bp

Spans 1 intron

NM_005022 · chr17

108 1 gene · 2 transcripts

FWD 5' GTCAACATCACGCCAGCTGA 3'

REV 5' TCACCGAACATTTCTGGCCC 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 100 bp

Spans 1 intron

NM_005022 · chr17

108 Specific

FWD 5' TGACAAGACGCTAGTCCTGC 3'

REV 5' AGACGAGGTCAGTACTGGGA 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001375991 · chr17

107 1 gene · 2 transcripts

FWD 5' CATCGTGGGCTACAAGGACT 3'

REV 5' CTTGACCGGTCTTTGCCAAC 3'

Fwd Tm 61.4°C

Rev Tm 61.3°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001375991 · chr17

107 1 gene · 2 transcripts

FWD 5' GTCAACATCACGCCAGCTGA 3'

REV 5' GTCCCGGATCACCGAACATT 3'

Fwd Tm 62.6°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001375991 · chr17

106 1 gene · 2 transcripts

FWD 5' ATCGTGGGCTACAAGGACTC 3'

REV 5' CAAGTGTCAGCCCATTCACG 3'

Fwd Tm 61.1°C

Rev Tm 61.1°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001375991 · chr17

106 1 gene · 2 transcripts

FWD 5' GTTCGTCAACATCACGCCAG 3'

REV 5' GTCCCGGATCACCGAACATT 3'

Fwd Tm 61.2°C

Rev Tm 61.9°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001375991 · chr17

106 1 gene · 2 transcripts

FWD 5' ATCGTGGGCTACAAGGACTC 3'

REV 5' GTCTTTGCCAACCAGGACAC 3'

Fwd Tm 61.1°C

Rev Tm 61.1°C

GC 55%

Product 102 bp

Spans 1 intron