PGK1 10 primer pairs found

Human

Show all (including non-specific)

NM_000291 · chrX

119 Specific

FWD 5' GACGTTAAAGGGAAGCGGGT 3'

REV 5' CGACTTGGCTCCATTGTCCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' GACGTTAAAGGGAAGCGGGT 3'

REV 5' ACCGACTTGGCTCCATTGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' ACGTTAAAGGGAAGCGGGTC 3'

REV 5' CGACTTGGCTCCATTGTCCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 134 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' ACGTTAAAGGGAAGCGGGTC 3'

REV 5' ACCGACTTGGCTCCATTGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 136 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' GTTAAAGGGAAGCGGGTCGT 3'

REV 5' CGACTTGGCTCCATTGTCCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' GTTAAAGGGAAGCGGGTCGT 3'

REV 5' ACCGACTTGGCTCCATTGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 134 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' TGGACGTTAAAGGGAAGCGG 3'

REV 5' CGACTTGGCTCCATTGTCCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' TGGACGTTAAAGGGAAGCGG 3'

REV 5' ACCGACTTGGCTCCATTGTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' GCTGGACAAGCTGGACGTTA 3'

REV 5' CGACTTGGCTCCATTGTCCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_000291 · chrX

119 Specific

FWD 5' GCTGGACAAGCTGGACGTTA 3'

REV 5' ACCGACTTGGCTCCATTGTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns