PPFIA3 10 primer pairs found

Human

Show all (including non-specific)

NM_003660 · chr19

119 Specific

FWD 5' ACGAGGAGATGGAGTCCCTT 3'

REV 5' CACCCACTCGTGGTTCATGT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 101 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' ACGAGGAGATGGAGTCCCTT 3'

REV 5' ACCCACTCGTGGTTCATGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 100 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' CTTCACCTCAAGGAGCGCAT 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' GAGCGCTTACAGCTTCACCT 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 138 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' AGCGCTTACAGCTTCACCTC 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 137 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' GCGCTTACAGCTTCACCTCA 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 136 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' AAGAACTCCCTGAGCGAGGA 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 90 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' AGTCCAACGAGCGCTTACAG 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_003660 · chr19

119 Specific

FWD 5' GAGTCCAACGAGCGCTTACA 3'

REV 5' CTCCATTTCGGCCAAGAGCT 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 147 bp

Spans 2 introns

NM_003660 · chr19

118 Specific

FWD 5' AAGAACTCCCTGAGCGAGGA 3'

REV 5' CCATCTGCATCCGCTCCATT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 103 bp

Spans 2 introns