PRKAA2 10 primer pairs found

Human

Show all (including non-specific)

NM_006252 · chr1

108 Specific

FWD 5' CCAGGTGATCAGCACTCCAA 3'

REV 5' GCCTGGCTTCCATCTCTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 113 bp

Spans 1 intron

NM_006252 · chr1

108 Specific

FWD 5' GCACCTTCGGCAAAGTGAAG 3'

REV 5' TTGGAGTGCTGATCACCTGG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 186 bp

Spans 2 introns

NM_006252 · chr1

106 Specific

FWD 5' CCAGGTGATCAGCACTCCAA 3'

REV 5' GAATCTGCTGAAAGAGCCGC 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 131 bp

Spans 1 intron

NM_006252 · chr1

106 Specific

FWD 5' ACCAGGTGATCAGCACTCCA 3'

REV 5' AATCTGCTGAAAGAGCCGCC 3'

Fwd Tm 62.9°C

Rev Tm 62.8°C

GC 55%

Product 131 bp

Spans 1 intron

NM_006252 · chr1

105 Specific

FWD 5' GCATGGACGGGTTGAAGAGA 3'

REV 5' TGTGTGCATCCAACAGGACA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 131 bp

Spans 1 intron

NM_006252 · chr1

105 Specific

FWD 5' AGCATGGACGGGTTGAAGAG 3'

REV 5' TGTGTGCATCCAACAGGACA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 1 intron

NM_006252 · chr1

105 Specific

FWD 5' ACAGGCCATAAAGTGGCAGT 3'

REV 5' TTGGAGTGCTGATCACCTGG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 50%

Product 148 bp

Spans 1 intron

NM_006252 · chr1

105 Specific

FWD 5' GGCACCTTCGGCAAAGTGAA 3'

REV 5' TGGAGTGCTGATCACCTGGT 3'

Fwd Tm 63.1°C

Rev Tm 62.9°C

GC 55%

Product 186 bp

Spans 2 introns

NM_006252 · chr1

105 Specific

FWD 5' CCGAAGATGGCTGAGAAGCA 3'

REV 5' ACTGCCACTTTATGGCCTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 137 bp

Spans 1 intron

NM_006252 · chr1

105 Specific

FWD 5' TACCAGGTGATCAGCACTCC 3'

REV 5' GAATCTGCTGAAAGAGCCGC 3'

Fwd Tm 60.8°C

Rev Tm 61.2°C

GC 55%

Product 133 bp

Spans 1 intron