PRPF18 10 primer pairs found

Human

Show all (including non-specific)

NM_003675 · chr10

117 Specific

FWD 5' AGGCGCTTGGAGAGTCCTTA 3'

REV 5' AGTTTACCCTGCACACTGCG 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_003675 · chr10

117 Specific

FWD 5' AGGCGCTTGGAGAGTCCTTA 3'

REV 5' TACCCTGCACACTGCGTTTC 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_003675 · chr10

117 Specific

FWD 5' AGGCGCTTGGAGAGTCCTTA 3'

REV 5' GTTTACCCTGCACACTGCGT 3'

Fwd Tm 62.6°C

Rev Tm 62.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_003675 · chr10

116 Specific

FWD 5' TTAGAGGCGCTTGGAGAGTC 3'

REV 5' CAGTTTACCCTGCACACTGC 3'

Fwd Tm 61.0°C

Rev Tm 61.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_003675 · chr10

115 Specific

FWD 5' GGCGCTTGGAGAGTCCTTAG 3'

REV 5' TACCCTGCACACTGCGTTTC 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 60%

Product 140 bp

Spans 2 introns

NM_003675 · chr10

115 Specific

FWD 5' GGCGCTTGGAGAGTCCTTAG 3'

REV 5' AGTTTACCCTGCACACTGCG 3'

Fwd Tm 62.1°C

Rev Tm 62.6°C

GC 60%

Product 144 bp

Spans 2 introns

NM_003675 · chr10

115 Specific

FWD 5' GGCGCTTGGAGAGTCCTTAG 3'

REV 5' TTACCCTGCACACTGCGTTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 60%

Product 141 bp

Spans 2 introns

NM_003675 · chr10

115 Specific

FWD 5' GGCGCTTGGAGAGTCCTTAG 3'

REV 5' TTTACCCTGCACACTGCGTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 60%

Product 142 bp

Spans 2 introns

NM_003675 · chr10

115 Specific

FWD 5' GTTAGAGGCGCTTGGAGAGT 3'

REV 5' TAGCCCAAACGCCAAGAAGA 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 98 bp

Spans 2 introns

NM_003675 · chr10

114 Specific

FWD 5' AGGCGCTTGGAGAGTCCTTA 3'

REV 5' AGCCCAAACGCCAAGAAGAA 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 92 bp

Spans 2 introns