PRPF8 20 primer pairs found

Human

Show all (including non-specific)

NM_006445 · chr17

119 Specific

FWD 5' TCCACCTCACCTGGTACCAT 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

119 Specific

FWD 5' TCCACCTCACCTGGTACCAT 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_006445 · chr17

116 Specific

FWD 5' CACCATGTCCACCTCACCTG 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 149 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

116 Specific

FWD 5' CACCATGTCCACCTCACCTG 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 149 bp

Spans 2 introns

NM_006445 · chr17

116 Specific

FWD 5' ACACCATGTCCACCTCACCT 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 63.0°C

Rev Tm 62.3°C

GC 55%

Product 150 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

116 Specific

FWD 5' ACACCATGTCCACCTCACCT 3'

REV 5' TCATCCGGCAATGGTTCCTG 3'

Fwd Tm 63.0°C

Rev Tm 62.3°C

GC 55%

Product 150 bp

Spans 2 introns

NM_006445 · chr17

115 Specific

FWD 5' ATGTCCACCTCACCTGGTAC 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 60.8°C

Rev Tm 61.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

115 Specific

FWD 5' ATGTCCACCTCACCTGGTAC 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 60.8°C

Rev Tm 61.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_006445 · chr17

115 Specific

FWD 5' CATGTCCACCTCACCTGGTA 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 60.8°C

Rev Tm 61.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

115 Specific

FWD 5' CATGTCCACCTCACCTGGTA 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 60.8°C

Rev Tm 61.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_006445 · chr17

114 Specific

FWD 5' TCCACCTCACCTGGTACCAT 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

114 Specific

FWD 5' TCCACCTCACCTGGTACCAT 3'

REV 5' CATCATCCGGCAATGGTTCC 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_006445 · chr17

114 Specific

FWD 5' GGATTCTCCGGATATGGCCG 3'

REV 5' GCCTGCAATTGCTGCCATTT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 60%

Product 138 bp

Spans 2 introns

NM_006445 · chr17

114 Specific

FWD 5' GATTCTCCGGATATGGCCGG 3'

REV 5' GCCTGCAATTGCTGCCATTT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 60%

Product 137 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

114 Specific

FWD 5' GGATTCTCCGGATATGGCCG 3'

REV 5' GCCTGCAATTGCTGCCATTT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 60%

Product 138 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

114 Specific

FWD 5' GATTCTCCGGATATGGCCGG 3'

REV 5' GCCTGCAATTGCTGCCATTT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 60%

Product 137 bp

Spans 2 introns

NM_006445 · chr17

114 Specific

FWD 5' TGGTATCGGGAGCATTGTCC 3'

REV 5' GGCTTTGAAGGAGCGGAACA 3'

Fwd Tm 61.5°C

Rev Tm 62.6°C

GC 55%

Product 147 bp

Spans 2 introns

NM_006445 · chr17

114 Specific

FWD 5' TGGTATCGGGAGCATTGTCC 3'

REV 5' TGGCTTTGAAGGAGCGGAAC 3'

Fwd Tm 61.5°C

Rev Tm 62.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

114 Specific

FWD 5' TGGTATCGGGAGCATTGTCC 3'

REV 5' GGCTTTGAAGGAGCGGAACA 3'

Fwd Tm 61.5°C

Rev Tm 62.6°C

GC 55%

Product 147 bp

Spans 2 introns

NM_006445 · chr17_KI270861v1_alt

114 Specific

FWD 5' TGGTATCGGGAGCATTGTCC 3'

REV 5' TGGCTTTGAAGGAGCGGAAC 3'

Fwd Tm 61.5°C

Rev Tm 62.6°C

GC 55%

Product 148 bp

Spans 2 introns