PYCARD 20 primer pairs found

Human

Show all (including non-specific)

NM_013258 · chr16

107 1 gene · 2 transcripts

FWD 5' GACATGGGCCTGCAGGAGAT 3'

REV 5' AGCCCGGTGCTGGTCTATAA 3'

Fwd Tm 64.2°C

Rev Tm 62.8°C

GC 60%

Product 138 bp

Spans 2 introns

NM_145182 · chr16

107 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' TCAGGACCTTCCCGTACAGA 3'

Fwd Tm 62.3°C

Rev Tm 61.7°C

GC 55%

Product 100 bp

Spans 1 intron

NM_145182 · chr16

107 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' CACTGCCTGGTACTGCTCAT 3'

Fwd Tm 62.3°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 1 intron

NM_013258 · chr16

106 Specific

FWD 5' AAGCCAGGCCTGCACTTTAT 3'

REV 5' TCAGGACCTTCCCGTACAGA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 50%

Product 100 bp

Spans 1 intron

NM_013258 · chr16

106 Specific

FWD 5' CAAGCCAGGCCTGCACTTTA 3'

REV 5' TGCTCATCCGTCAGGACCTT 3'

Fwd Tm 62.7°C

Rev Tm 62.9°C

GC 55%

Product 111 bp

Spans 1 intron

NM_145182 · chr16

106 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' CTGCCTGGTACTGCTCATCC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 121 bp

Spans 1 intron

NM_145182 · chr16

106 1 gene · 2 transcripts

FWD 5' TCTACCTGGAGACCTACGGC 3'

REV 5' TTGTGACCCTCGCGATAAGC 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 60%

Product 150 bp

Spans 1 intron

NM_145182 · chr16

106 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' GCTCATCCGTCAGGACCTTC 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 1 intron

NM_013258 · chr16

106 1 gene · 2 transcripts

FWD 5' ACATGGGCCTGCAGGAGATG 3'

REV 5' AGCCCGGTGCTGGTCTATAA 3'

Fwd Tm 64.5°C

Rev Tm 62.8°C

GC 60%

Product 137 bp

Spans 2 introns

NM_013258 · chr16

106 Specific

FWD 5' AAGCCAGGCCTGCACTTTAT 3'

REV 5' CACTGCCTGGTACTGCTCAT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 50%

Product 123 bp

Spans 1 intron

NM_145182 · chr16

106 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' TGCTCATCCGTCAGGACCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.9°C

GC 55%

Product 110 bp

Spans 1 intron

NM_013258 · chr16

106 1 gene · 2 transcripts

FWD 5' AGCTGGTCAGCTTCTACCTG 3'

REV 5' GCCCGGTGCTGGTCTATAAA 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 55%

Product 196 bp

Spans 2 introns

NM_145182 · chr16

106 1 gene · 2 transcripts

FWD 5' AGCTGGTCAGCTTCTACCTG 3'

REV 5' GCCCGGTGCTGGTCTATAAA 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 55%

Product 139 bp

Spans 1 intron

NM_013258 · chr16

106 1 gene · 2 transcripts

FWD 5' GACATGGGCCTGCAGGAGAT 3'

REV 5' CAGCCCGGTGCTGGTCTATA 3'

Fwd Tm 64.2°C

Rev Tm 63.5°C

GC 60%

Product 139 bp

Spans 2 introns

NM_013258 · chr16

105 1 gene · 2 transcripts

FWD 5' GCTGGTCAGCTTCTACCTGG 3'

REV 5' GCCCGGTGCTGGTCTATAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 60%

Product 195 bp

Spans 2 introns

NM_145182 · chr16

105 1 gene · 2 transcripts

FWD 5' GCTGGTCAGCTTCTACCTGG 3'

REV 5' GCCCGGTGCTGGTCTATAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 60%

Product 138 bp

Spans 1 intron

NM_145182 · chr16

105 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' CCTTCCCGTACAGAGCATCC 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 94 bp

Spans 1 intron

NM_145182 · chr16

105 Specific

FWD 5' CACCAGGGCCTGCACTTTAT 3'

REV 5' ACTGCCTGGTACTGCTCATC 3'

Fwd Tm 62.3°C

Rev Tm 61.4°C

GC 55%

Product 122 bp

Spans 1 intron

NM_013258 · chr16

105 1 gene · 2 transcripts

FWD 5' GACATGGGCCTGCAGGAGAT 3'

REV 5' GCCCGGTGCTGGTCTATAAA 3'

Fwd Tm 64.2°C

Rev Tm 61.7°C

GC 60%

Product 137 bp

Spans 2 introns

NM_013258 · chr16

105 Specific

FWD 5' CAAGCCAGGCCTGCACTTTA 3'

REV 5' TCAGGACCTTCCCGTACAGA 3'

Fwd Tm 62.7°C

Rev Tm 61.7°C

GC 55%

Product 101 bp

Spans 1 intron