Phka2 10 primer pairs found

Human

Show all (including non-specific)

NM_000292 · chrX

119 Specific

FWD 5' AGGGTGTGGAAGATCCTCCA 3'

REV 5' ACCGATTCGACATGGACAGC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 122 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' AGGGTGTGGAAGATCCTCCA 3'

REV 5' AGCACCGATTCGACATGGAC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' AGGGTGTGGAAGATCCTCCA 3'

REV 5' CCGATTCGACATGGACAGCA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' AGGGTGTGGAAGATCCTCCA 3'

REV 5' TCAGCACCGATTCGACATGG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' CTGGAGCAGAACGTGGTGAA 3'

REV 5' CCTTGGTGCTCTGAGTGTGT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 100 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' TGGAGCAGAACGTGGTGAAG 3'

REV 5' CCTTGGTGCTCTGAGTGTGT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 99 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' AGGGTGTGGAAGATCCTCCA 3'

REV 5' GGTTCAGCACCGATTCGACA 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 130 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' GCCATACCGGAGTCACCAAA 3'

REV 5' TCGCAGACTTGGAGGAATGC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' GAGCAGAACGTGGTGAAGCT 3'

REV 5' AGGTGGCGGTGTTGTACTTG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 130 bp

Spans 2 introns

NM_000292 · chrX

119 Specific

FWD 5' GAGCAGAACGTGGTGAAGCT 3'

REV 5' CAGGTGGCGGTGTTGTACTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 2 introns