Piga 20 primer pairs found

Human

Show all (including non-specific)

NM_020473 · chrX

110 Specific

FWD 5' CTCACCGGGATCGATTTGCT 3'

REV 5' TGCAGCTGGTATCTTTCCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 134 bp

Spans 1 intron

NM_002641 · chrX

110 1 gene · 2 transcripts

FWD 5' CGGGAAAGATACCAGCTGCA 3'

REV 5' GCACCTCAGGAATTCCACCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 196 bp

Spans 2 introns

NM_020473 · chrX

110 1 gene · 2 transcripts

FWD 5' CGGGAAAGATACCAGCTGCA 3'

REV 5' GCACCTCAGGAATTCCACCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 196 bp

Spans 2 introns

NM_020473 · chrX

109 Specific

FWD 5' CTCACCGGGATCGATTTGCT 3'

REV 5' TCTTTGGTCCCTCTCCTCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 94 bp

Spans 1 intron

NM_020473 · chrX

109 Specific

FWD 5' CTCACCGGGATCGATTTGCT 3'

REV 5' CCCTGTCATGCAGCTGGTAT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Spans 1 intron

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' GCACCTCAGGAATTCCACCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 188 bp

Spans 2 introns

NM_020473 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' GCACCTCAGGAATTCCACCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 188 bp

Spans 2 introns

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' CGCCATGCAGAATGCTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 121 bp

Spans 1 intron

NM_020473 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' CGCCATGCAGAATGCTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 121 bp

Spans 1 intron

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' CGGGAAAGATACCAGCTGCA 3'

REV 5' CGCCATGCAGAATGCTTCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 129 bp

Spans 1 intron

NM_020473 · chrX

109 1 gene · 2 transcripts

FWD 5' CGGGAAAGATACCAGCTGCA 3'

REV 5' CGCCATGCAGAATGCTTCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 129 bp

Spans 1 intron

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' AGCACCTCAGGAATTCCACC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 189 bp

Spans 2 introns

NM_020473 · chrX

109 1 gene · 2 transcripts

FWD 5' ATACCAGCTGCATGACAGGG 3'

REV 5' AGCACCTCAGGAATTCCACC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 189 bp

Spans 2 introns

NM_020473 · chrX

109 Specific

FWD 5' ACTCACCGGGATCGATTTGC 3'

REV 5' TGCAGCTGGTATCTTTCCCG 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 135 bp

Spans 1 intron

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' TCGGGAAAGATACCAGCTGC 3'

REV 5' AGCACCTCAGGAATTCCACC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 198 bp

Spans 2 introns

NM_020473 · chrX

109 1 gene · 2 transcripts

FWD 5' TCGGGAAAGATACCAGCTGC 3'

REV 5' AGCACCTCAGGAATTCCACC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 198 bp

Spans 2 introns

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' CGGGAAAGATACCAGCTGCA 3'

REV 5' AGCACCTCAGGAATTCCACC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 197 bp

Spans 2 introns

NM_002641 · chrX

109 1 gene · 2 transcripts

FWD 5' TCGGGAAAGATACCAGCTGC 3'

REV 5' CGCCATGCAGAATGCTTCAG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 130 bp

Spans 1 intron

NM_002641 · chrX

109 Specific

FWD 5' GCCATGGAACTCACCGGTAA 3'

REV 5' ACCCGAGAGAGTGTAGCTGA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 1 intron

NM_002641 · chrX

108 1 gene · 2 transcripts

FWD 5' TCGGGAAAGATACCAGCTGC 3'

REV 5' GCACCTCAGGAATTCCACCA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 197 bp

Spans 2 introns