Pole4 10 primer pairs found

Human

Show all (including non-specific)

NM_019896 · chr2

119 Specific

FWD 5' AGATGCCTACTGTTGCGCTC 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_019896 · chr2

118 Specific

FWD 5' GATGCCTACTGTTGCGCTCA 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 126 bp

Spans 2 introns

NM_019896 · chr2

118 Specific

FWD 5' ATGCCTACTGTTGCGCTCAG 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 125 bp

Spans 2 introns

NM_019896 · chr2

116 Specific

FWD 5' AAGATGCCTACTGTTGCGCT 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 50%

Product 128 bp

Spans 2 introns

NM_019896 · chr2

112 Specific

FWD 5' CCTACTGTTGCGCTCAGCAG 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 63.0°C

Rev Tm 62.0°C

GC 60%

Product 122 bp

Spans 2 introns

NM_019896 · chr2

112 Specific

FWD 5' CTACTGTTGCGCTCAGCAGG 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 63.0°C

Rev Tm 62.0°C

GC 60%

Product 121 bp

Spans 2 introns

NM_019896 · chr2

111 Specific

FWD 5' AAAGATGCCTACTGTTGCGC 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 60.6°C

Rev Tm 62.0°C

GC 50%

Product 129 bp

Spans 2 introns

NM_019896 · chr2

109 Specific

FWD 5' CGAGTGAAGGCCTTGGTGAA 3'

REV 5' GAGCGCAACAGTAGGCATCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_019896 · chr2

109 Specific

FWD 5' TCTTCATTCTGGCACGAGCC 3'

REV 5' CCCGCTCGGCAATCAATCTA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 176 bp

Spans 3 introns

NM_019896 · chr2

109 Specific

FWD 5' AGTGAAGGCCTTGGTGAAGG 3'

REV 5' GAGCGCAACAGTAGGCATCT 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 1 intron