Polr2a 10 primer pairs found

Human

Show all (including non-specific)

NM_000937 · chr17

117 Specific

FWD 5' ACAACTGGCTCCTCATCGAG 3'

REV 5' CCAGCTCATTGTTGTGTGCC 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_000937 · chr17

116 Specific

FWD 5' TGCAAGAGGAGGAAGAGGTG 3'

REV 5' CTGTCTGTGGCAAGTGCATG 3'

Fwd Tm 61.0°C

Rev Tm 61.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_000937 · chr17

115 Specific

FWD 5' CAACTGGCTCCTCATCGAGG 3'

REV 5' CCAGCTCATTGTTGTGTGCC 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 60%

Product 146 bp

Spans 2 introns

NM_000937 · chr17

115 Specific

FWD 5' GAGGAGGAAGAGGTGGTGGA 3'

REV 5' GTCTGTCTGTGGCAAGTGCA 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 60%

Product 135 bp

Spans 2 introns

NM_000937 · chr17

115 Specific

FWD 5' GAGGAGGAAGAGGTGGTGGA 3'

REV 5' TGTCTGTCTGTGGCAAGTGC 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 60%

Product 136 bp

Spans 2 introns

NM_000937 · chr17

115 Specific

FWD 5' AGAGGAGGAAGAGGTGGTGG 3'

REV 5' GTCTGTCTGTGGCAAGTGCA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 60%

Product 136 bp

Spans 2 introns

NM_000937 · chr17

115 Specific

FWD 5' AGAGGAGGAAGAGGTGGTGG 3'

REV 5' TGTCTGTCTGTGGCAAGTGC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 60%

Product 137 bp

Spans 2 introns

NM_000937 · chr17

114 Specific

FWD 5' GCAAGAGGAGGAAGAGGTGG 3'

REV 5' CTGTCTGTGGCAAGTGCATG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 60%

Product 137 bp

Spans 2 introns

NM_000937 · chr17

114 Specific

FWD 5' GCAAGAGGAGGAAGAGGTGG 3'

REV 5' GTCTGTCTGTGGCAAGTGCA 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 60%

Product 139 bp

Spans 2 introns

NM_000937 · chr17

114 Specific

FWD 5' GCAAGAGGAGGAAGAGGTGG 3'

REV 5' TGTCTGTCTGTGGCAAGTGC 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 60%

Product 140 bp

Spans 2 introns