Ppia 20 primer pairs found

Human

Show all (including non-specific)

NM_021130 · chr7

119 1 gene · 2 transcripts

FWD 5' CCCACCGTGTTCTTCGACAT 3'

REV 5' TCTCTCCAGTGCTCAGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 121 bp

Spans 2 introns

NM_021130 · chr7

117 Specific

FWD 5' GCTGTTTGCAGACAAGGTCC 3'

REV 5' GAAGTCACCACCCTGACACA 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 133 bp

Spans 2 introns

NM_021130 · chr7

117 Specific

FWD 5' GCTGTTTGCAGACAAGGTCC 3'

REV 5' TGAAGTCACCACCCTGACAC 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_021130 · chr7

117 Specific

FWD 5' GCTGTTTGCAGACAAGGTCC 3'

REV 5' GTGAAGTCACCACCCTGACA 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_021130 · chr7

117 Specific

FWD 5' GCTGTTTGCAGACAAGGTCC 3'

REV 5' TGTGAAGTCACCACCCTGAC 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_021130 · chr7

117 Specific

FWD 5' GCTGTTTGCAGACAAGGTCC 3'

REV 5' GTGTGAAGTCACCACCCTGA 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001300981 · chr7

116 Specific

FWD 5' AGCAGTGCTTGCTGTTCCTT 3'

REV 5' TCTCTCCAGTGCTCAGAGCA 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 50%

Product 94 bp

Spans 2 introns

NM_021130 · chr7

116 1 gene · 2 transcripts

FWD 5' CGTGTTCTTCGACATTGCCG 3'

REV 5' TCTCTCCAGTGCTCAGAGCA 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 116 bp

Spans 2 introns

NM_001300981 · chr7

116 Specific

FWD 5' CTGATCGTGCAGCAGTGCTT 3'

REV 5' TCTCTCCAGTGCTCAGAGCA 3'

Fwd Tm 62.9°C

Rev Tm 62.3°C

GC 55%

Product 104 bp

Spans 2 introns

NM_021130 · chr7

116 1 gene · 2 transcripts

FWD 5' GTTCTTCGACATTGCCGTCG 3'

REV 5' TTCTCTCCAGTGCTCAGAGC 3'

Fwd Tm 61.2°C

Rev Tm 61.0°C

GC 55%

Product 114 bp

Spans 2 introns

NM_021130 · chr7

116 1 gene · 2 transcripts

FWD 5' CCGTGTTCTTCGACATTGCC 3'

REV 5' TTCTCTCCAGTGCTCAGAGC 3'

Fwd Tm 61.3°C

Rev Tm 61.0°C

GC 55%

Product 118 bp

Spans 2 introns

NM_021130 · chr7

115 1 gene · 2 transcripts

FWD 5' CGTGTTCTTCGACATTGCCG 3'

REV 5' TTCTCTCCAGTGCTCAGAGC 3'

Fwd Tm 61.5°C

Rev Tm 61.0°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001300981 · chr7

115 1 gene · 2 transcripts

FWD 5' TGTTTGCAGACAAGGTCCCA 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001300981 · chr7

115 Specific

FWD 5' TGATCGTGCAGCAGTGCTTG 3'

REV 5' TCTCTCCAGTGCTCAGAGCA 3'

Fwd Tm 63.1°C

Rev Tm 62.3°C

GC 55%

Product 103 bp

Spans 2 introns

NM_001300981 · chr7

115 1 gene · 2 transcripts

FWD 5' ACAAGGTCCCAAAGACAGCA 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 50%

Product 141 bp

Spans 2 introns

NM_001300981 · chr7

113 1 gene · 2 transcripts

FWD 5' CAAGGTCCCAAAGACAGCAG 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 60.6°C

Rev Tm 61.5°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001300981 · chr7

113 1 gene · 2 transcripts

FWD 5' GCAGACAAGGTCCCAAAGAC 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 60.5°C

Rev Tm 61.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001300981 · chr7

113 1 gene · 2 transcripts

FWD 5' GACAAGGTCCCAAAGACAGC 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 60.5°C

Rev Tm 61.5°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001300981 · chr7

113 1 gene · 2 transcripts

FWD 5' TGCAGACAAGGTCCCAAAGA 3'

REV 5' CAGTGCCATTATGGCGTGTG 3'

Fwd Tm 61.0°C

Rev Tm 61.5°C

GC 50%

Product 146 bp

Spans 2 introns

NM_001300981 · chr7

113 1 gene · 2 transcripts

FWD 5' TGTTTGCAGACAAGGTCCCA 3'

REV 5' AGTGCCATTATGGCGTGTGA 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 50%

Product 149 bp

Spans 2 introns