Prok1 10 primer pairs found

Human

Show all (including non-specific)

NM_032414 · chr1

108 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' GGTGCTTGCGTTTCCTGAAG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 174 bp

Spans 2 introns

NM_032414 · chr1

107 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' AAGGACAGGTGTGGTGCTTG 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 186 bp

Spans 2 introns

NM_032414 · chr1

107 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' CAAGGACAGGTGTGGTGCTT 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 187 bp

Spans 2 introns

NM_032414 · chr1

107 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' AAGCAAGGACAGGTGTGGTG 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 190 bp

Spans 2 introns

NM_032414 · chr1

107 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' CAAGCAAGGACAGGTGTGGT 3'

Fwd Tm 61.8°C

Rev Tm 62.4°C

GC 55%

Product 191 bp

Spans 2 introns

NM_032414 · chr1

105 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' TGGTGCTTGCGTTTCCTGAA 3'

Fwd Tm 61.8°C

Rev Tm 62.3°C

GC 55%

Product 175 bp

Spans 2 introns

NM_032414 · chr1

104 Specific

FWD 5' ACTGTGCTGTGATCACAGGG 3'

REV 5' GTGGTGCTTGCGTTTCCTGA 3'

Fwd Tm 61.8°C

Rev Tm 63.1°C

GC 55%

Product 176 bp

Spans 2 introns

NM_032414 · chr1

104 Specific

FWD 5' AGTGACCATGAGAGGTGCCA 3'

REV 5' CTCGAAGCCACAGGCTGATG 3'

Fwd Tm 62.9°C

Rev Tm 62.9°C

GC 55%

Product 143 bp

Spans 1 intron

NM_032414 · chr1

103 Specific

FWD 5' GCCACGCGAGTCTCAATCAT 3'

REV 5' CTCGAAGCCACAGGCTGATG 3'

Fwd Tm 62.4°C

Rev Tm 62.9°C

GC 55%

Product 127 bp

Spans 1 intron

NM_032414 · chr1

103 Specific

FWD 5' CATCAGCCTGTGGCTTCGAG 3'

REV 5' AAGGACAGGTGTGGTGCTTG 3'

Fwd Tm 62.9°C

Rev Tm 62.4°C

GC 60%

Product 122 bp

Spans 1 intron