RFFL 10 primer pairs found

Human

Show all (including non-specific)

NM_001017368 · chr17

119 Specific

FWD 5' AGTGGGAGCTGATGGAGAGA 3'

REV 5' CCAAGCCTGATGGTACTGCT 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 111 bp

Spans 2 introns

NM_001017368 · chr17

119 Specific

FWD 5' TGGGAGCTGATGGAGAGAGT 3'

REV 5' CCAAGCCTGATGGTACTGCT 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 2 introns

NM_001017368 · chr17

119 Specific

FWD 5' AGTGGGAGCTGATGGAGAGA 3'

REV 5' TCCAAGCCTGATGGTACTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001017368 · chr17

119 Specific

FWD 5' TGGGAGCTGATGGAGAGAGT 3'

REV 5' TCCAAGCCTGATGGTACTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 110 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' ACTACAAGGGCTGCTGTGAG 3'

REV 5' ACACAGGTTCTCCTCCAAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' ACTACAAGGGCTGCTGTGAG 3'

REV 5' TCCAAGCCTGATGGTACTGC 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' ACTACAAGGGCTGCTGTGAG 3'

REV 5' CCAAGCCTGATGGTACTGCT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' ACAAGGGCTGCTGTGAGAAG 3'

REV 5' AGGTTCTCCTCCAAGCCTGA 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' CAAGGGCTGCTGTGAGAAGT 3'

REV 5' AGGTTCTCCTCCAAGCCTGA 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001017368 · chr17

118 Specific

FWD 5' AAGGGCTGCTGTGAGAAGTG 3'

REV 5' AGGTTCTCCTCCAAGCCTGA 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 137 bp

Spans 2 introns