RFT1 10 primer pairs found

Human

Show all (including non-specific)

NM_052859 · chr3

119 Specific

FWD 5' GGTGATTGCAGAGAGCCTGT 3'

REV 5' AGCAGAGCACCAGAACTGTG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 134 bp

Spans 2 introns

NM_052859 · chr3

119 Specific

FWD 5' GGTGATTGCAGAGAGCCTGT 3'

REV 5' CAGAGCACCAGAACTGTGGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 132 bp

Spans 2 introns

NM_052859 · chr3

119 Specific

FWD 5' AGGTGATTGCAGAGAGCCTG 3'

REV 5' CAGAGCACCAGAACTGTGGT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_052859 · chr3

118 Specific

FWD 5' AGGTGATTGCAGAGAGCCTG 3'

REV 5' AGCAGAGCACCAGAACTGTG 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 2 introns

NM_052859 · chr3

117 Specific

FWD 5' CTCCTCCTGCAGGTGTTGTT 3'

REV 5' CCAGGAAGAGGGTGGTTGAG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 133 bp

Spans 2 introns

NM_052859 · chr3

116 Specific

FWD 5' CTCCTGCAGGTGTTGTTTCG 3'

REV 5' CAGGAAGAGGGTGGTTGAGT 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 129 bp

Spans 2 introns

NM_052859 · chr3

116 Specific

FWD 5' CTCCTGCAGGTGTTGTTTCG 3'

REV 5' GGTGGTTGAGTAAAGCAGCG 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 120 bp

Spans 2 introns

NM_052859 · chr3

116 Specific

FWD 5' TTAGCTCAGGATCCGGTCCT 3'

REV 5' GACAGGGCCAGCATCACAAA 3'

Fwd Tm 61.9°C

Rev Tm 62.7°C

GC 55%

Product 148 bp

Spans 2 introns

NM_052859 · chr3

116 Specific

FWD 5' TTTGACAGAAGGCGAGCGAT 3'

REV 5' TAATCTGGCCACAAGGGAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 50%

Product 109 bp

Spans 2 introns

NM_052859 · chr3

115 Specific

FWD 5' CTCCTCCTGCAGGTGTTGTT 3'

REV 5' GGTGGTTGAGTAAAGCAGCG 3'

Fwd Tm 61.8°C

Rev Tm 61.0°C

GC 55%

Product 123 bp

Spans 2 introns