RPE65 10 primer pairs found

Human

Show all (including non-specific)

NM_000329 · chr1

120 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' CAAAGAGTCCTGGCCCACAT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 2 introns

NM_000329 · chr1

119 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' TTCAAAGAGTCCTGGCCCAC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NM_000329 · chr1

118 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' AAAGAGTCCTGGCCCACATC 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 144 bp

Spans 2 introns

NM_000329 · chr1

118 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' ACTTCAAAGAGTCCTGGCCC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_000329 · chr1

118 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' CTTCAAAGAGTCCTGGCCCA 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_000329 · chr1

116 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' CCACATCGAAGGAGACTGCC 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 131 bp

Spans 2 introns

NM_000329 · chr1

116 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' CCCACATCGAAGGAGACTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 132 bp

Spans 2 introns

NM_000329 · chr1

116 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' GCCCACATCGAAGGAGACTG 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 133 bp

Spans 2 introns

NM_000329 · chr1

115 Specific

FWD 5' ATCCAGGTTGAGCATCCTGC 3'

REV 5' CACATCGAAGGAGACTGCCG 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 130 bp

Spans 2 introns

NM_000329 · chr1

115 Specific

FWD 5' TCCAGGTTGAGCATCCTGCT 3'

REV 5' TCAAAGAGTCCTGGCCCACA 3'

Fwd Tm 63.3°C

Rev Tm 63.2°C

GC 55%

Product 145 bp

Spans 2 introns