RTF1 10 primer pairs found

Human

Show all (including non-specific)

NM_015138 · chr15

119 Specific

FWD 5' GGAACCGGGAGTGGAACATT 3'

REV 5' GCTTGAACAGCTGGGTCTCT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 145 bp

Spans 2 introns

NM_015138 · chr15

119 Specific

FWD 5' GGAACCGGGAGTGGAACATT 3'

REV 5' AGCTTGAACAGCTGGGTCTC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 2 introns

NM_015138 · chr15

119 Specific

FWD 5' GCATCGGAAACCACAACAGC 3'

REV 5' GGAACACGCGTTGGTCATTG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' GTTTACCGGGTCGCTGAGAT 3'

REV 5' GGAACACGCGTTGGTCATTG 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 118 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' GCATCGGAAACCACAACAGC 3'

REV 5' GAACACGCGTTGGTCATTGC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' CTACGGCATGGCAATGACCA 3'

REV 5' TAGAGTGGGCAACTGCATGC 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 126 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' ACTACGGCATGGCAATGACC 3'

REV 5' TAGAGTGGGCAACTGCATGC 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 127 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' GGAACCGGGAGTGGAACATT 3'

REV 5' TTGAACAGCTGGGTCTCTGG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' GCAACTACGGCATGGCAATG 3'

REV 5' TAGAGTGGGCAACTGCATGC 3'

Fwd Tm 62.0°C

Rev Tm 62.5°C

GC 55%

Product 130 bp

Spans 2 introns

NM_015138 · chr15

118 Specific

FWD 5' GTTTACCGGGTCGCTGAGAT 3'

REV 5' CTAAACGGAACACGCGTTGG 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 124 bp

Spans 2 introns