SEMA5A 10 primer pairs found

Human

Show all (including non-specific)

NM_003966 · chr5

117 Specific

FWD 5' GGCCGTGTGTGTTTGACTCT 3'

REV 5' CACGGCGATCATGTGGAACA 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 110 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' CGGGAGTTCAGAGCGAAGAA 3'

REV 5' TCACACTCCCATTCCACAGC 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' GCACCCGGATGTAGTTCTGA 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 98 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' AGCACCCGGATGTAGTTCTG 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 99 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' CAGAAGCACCCGGATGTAGT 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 103 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' TCCCACAGGTGAATAACCGG 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 134 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' CGTGAATGCATTGGTCCCAC 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 148 bp

Spans 2 introns

NM_003966 · chr5

117 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' CCCACAGGTGAATAACCGGT 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 2 introns

NM_003966 · chr5

116 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' ACAGGTGAATAACCGGTCGC 3'

Fwd Tm 61.3°C

Rev Tm 62.1°C

GC 55%

Product 130 bp

Spans 2 introns

NM_003966 · chr5

116 Specific

FWD 5' TATCCAGGCTGTGGAATGGG 3'

REV 5' GAATGCATTGGTCCCACAGG 3'

Fwd Tm 61.3°C

Rev Tm 61.0°C

GC 55%

Product 145 bp

Spans 2 introns