SHARPIN 10 primer pairs found

Human

Show all (including non-specific)

NM_030974 · chr8

113 Specific

FWD 5' GGCTGCAGGTCACACTTGAA 3'

REV 5' GGGAAACCGAGCTCTGAGAA 3'

Fwd Tm 62.6°C

Rev Tm 61.3°C

GC 55%

Product 133 bp

Spans 2 introns

NM_030974 · chr8

111 Specific

FWD 5' ATCAGGCTGCAGGTCACACT 3'

REV 5' CGGGAAACCGAGCTCTGAGA 3'

Fwd Tm 63.5°C

Rev Tm 63.5°C

GC 55%

Product 138 bp

Spans 2 introns

NM_030974 · chr8

111 Specific

FWD 5' TCAGGCTGCAGGTCACACTT 3'

REV 5' CGGGAAACCGAGCTCTGAGA 3'

Fwd Tm 63.6°C

Rev Tm 63.5°C

GC 55%

Product 137 bp

Spans 2 introns

NM_030974 · chr8

111 Specific

FWD 5' AGGCTGCAGGTCACACTTGA 3'

REV 5' CGGGAAACCGAGCTCTGAGA 3'

Fwd Tm 63.6°C

Rev Tm 63.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_030974 · chr8

110 Specific

FWD 5' GGCTGCAGGTCACACTTGAA 3'

REV 5' CGGGAAACCGAGCTCTGAGA 3'

Fwd Tm 62.6°C

Rev Tm 63.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_030974 · chr8

109 Specific

FWD 5' TCCTAGGAGCCCTGGAAACT 3'

REV 5' GCCTCCTGAAGCTGAACACT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 1 intron

NM_030974 · chr8

109 Specific

FWD 5' TCCTAGGAGCCCTGGAAACT 3'

REV 5' TGAAGCTGAACACTCAGGGC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 1 intron

NM_030974 · chr8

109 Specific

FWD 5' CGTGGAAGGACAGAATGGCA 3'

REV 5' AGTTTCCAGGGCTCCTAGGA 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 1 intron

NM_030974 · chr8

109 Specific

FWD 5' ATCAGGCTGCAGGTCACACT 3'

REV 5' GGGAAACCGAGCTCTGAGAA 3'

Fwd Tm 63.5°C

Rev Tm 61.3°C

GC 55%

Product 137 bp

Spans 2 introns

NM_030974 · chr8

109 Specific

FWD 5' CAGGCTGCAGGTCACACTTG 3'

REV 5' CGGGAAACCGAGCTCTGAGA 3'

Fwd Tm 63.4°C

Rev Tm 63.5°C

GC 60%

Product 136 bp

Spans 2 introns