SHH 20 primer pairs found

Human

Show all (including non-specific)

NM_001310462 · chr7

109 Specific

FWD 5' GCACATATCCACTGCTCGGT 3'

REV 5' AACTCTTGGCTCCGTCAACC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001310462 · chr7

109 Specific

FWD 5' GCACATATCCACTGCTCGGT 3'

REV 5' GCTCCGTCAACCCTGAATGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001310462 · chr7

109 Specific

FWD 5' AGGCACATATCCACTGCTCG 3'

REV 5' GCTCCGTCAACCCTGAATGA 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' AGGCACATATCCACTGCTCG 3'

REV 5' ACCCTGAATGAGAAGTCGGC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' CCCGAGCCGCTACACTAAAT 3'

REV 5' AGGTCCTGAAACAGCCGATG 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' CCCGAGCCGCTACACTAAAT 3'

REV 5' CAGGTCCTGAAACAGCCGAT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' GCACATATCCACTGCTCGGT 3'

REV 5' ACCCTGAATGAGAAGTCGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' AGGCACATATCCACTGCTCG 3'

REV 5' AACTCTTGGCTCCGTCAACC 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_000193 · chr7

108 Specific

FWD 5' AGGCACATATCCACTGCTCG 3'

REV 5' TCAGGTCCTTCACCAGCTTG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' ATCCACTGCTCGGTGAAAGC 3'

REV 5' AACTCTTGGCTCCGTCAACC 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 1 intron

NM_000193 · chr7

108 Specific

FWD 5' CTGCTCGGTGAAAGCAGAGA 3'

REV 5' TCAGGTCCTTCACCAGCTTG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 110 bp

Spans 1 intron

NM_000193 · chr7

108 Specific

FWD 5' GCACATATCCACTGCTCGGT 3'

REV 5' TCAGGTCCTTCACCAGCTTG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001310462 · chr7

108 Specific

FWD 5' GCAGGCAAGGAAAGGAAACC 3'

REV 5' ACCCTGAATGAGAAGTCGGC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 104 bp

Spans 1 intron

NM_000193 · chr7

108 Specific

FWD 5' TCTCCAGAAACTCCGAGCGA 3'

REV 5' ATCACCGAGATGGCCAAAGC 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 145 bp

Spans 1 intron

NM_000193 · chr7

108 Specific

FWD 5' ACTGCTCGGTGAAAGCAGAG 3'

REV 5' TCAGGTCCTTCACCAGCTTG 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 111 bp

Spans 1 intron

NM_000193 · chr7

107 Specific

FWD 5' TCTCCAGAAACTCCGAGCGA 3'

REV 5' GTTCATCACCGAGATGGCCA 3'

Fwd Tm 62.5°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 1 intron

NM_000193 · chr7

107 Specific

FWD 5' AGGCACATATCCACTGCTCG 3'

REV 5' CTCAGGTCCTTCACCAGCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.3°C

GC 55%

Product 124 bp

Spans 1 intron

NM_000193 · chr7

107 Specific

FWD 5' CTGCTCGGTGAAAGCAGAGA 3'

REV 5' CTCAGGTCCTTCACCAGCTT 3'

Fwd Tm 61.8°C

Rev Tm 61.3°C

GC 55%

Product 111 bp

Spans 1 intron

NM_000193 · chr7

107 Specific

FWD 5' GCACATATCCACTGCTCGGT 3'

REV 5' CTCAGGTCCTTCACCAGCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.3°C

GC 55%

Product 122 bp

Spans 1 intron

NM_000193 · chr7

107 Specific

FWD 5' CACTGCTCGGTGAAAGCAGA 3'

REV 5' TCAGGTCCTTCACCAGCTTG 3'

Fwd Tm 62.3°C

Rev Tm 61.6°C

GC 55%

Product 112 bp

Spans 1 intron