No gene-specific primers found for SIK1. Showing all 20 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

SIK1 20 primer pairs found

Human

Show all (including non-specific)

NM_173354 · chr21

119 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 148 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

119 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 148 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

116 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' GCATCCACCGGGAAGAACAA 3'

Fwd Tm 61.6°C

Rev Tm 62.5°C

GC 55%

Product 150 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

116 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' GCATCCACCGGGAAGAACAA 3'

Fwd Tm 61.6°C

Rev Tm 62.5°C

GC 55%

Product 150 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

115 2 genes · 2 transcripts

FWD 5' AGGTGCCTCAGGAAGGTCTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 62.7°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

115 2 genes · 2 transcripts

FWD 5' AGGTGCCTCAGGAAGGTCTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 62.7°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

112 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' CATCCACCGGGAAGAACAAG 3'

Fwd Tm 61.6°C

Rev Tm 60.4°C

GC 55%

Product 149 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

112 2 genes · 2 transcripts

FWD 5' GGTGCCTCAGGAAGGTCTTT 3'

REV 5' CATCCACCGGGAAGAACAAG 3'

Fwd Tm 61.6°C

Rev Tm 60.4°C

GC 55%

Product 149 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

110 2 genes · 2 transcripts

FWD 5' GAGGTGCCTCAGGAAGGTCT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 63.2°C

Rev Tm 61.7°C

GC 60%

Product 150 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

110 2 genes · 2 transcripts

FWD 5' GAGGTGCCTCAGGAAGGTCT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 63.2°C

Rev Tm 61.7°C

GC 60%

Product 150 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' AGTATCGGAATGCCCAGTGC 3'

REV 5' AAAGGGTCGGTGGAAAGACC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 112 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' AGTATCGGAATGCCCAGTGC 3'

REV 5' AAAGGGTCGGTGGAAAGACC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 112 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' GACGGTGGAGTCACTGCAAA 3'

REV 5' GCACTGGGCATTCCGATACT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' GACGGTGGAGTCACTGCAAA 3'

REV 5' GCACTGGGCATTCCGATACT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' CACTCACCGCGCCATGTATA 3'

REV 5' TTGCTCGCAGAGAAGGTCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' CACTCACCGCGCCATGTATA 3'

REV 5' TTGCTCGCAGAGAAGGTCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' GGTCTTTCCACCGACCCTTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 135 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' GGTCTTTCCACCGACCCTTT 3'

REV 5' ATCCACCGGGAAGAACAAGG 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 135 bp

Spans 1 intron

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' TCGTGAGGTTCAGCTGATGA 3'

REV 5' GTGCCCGTTGGAAGTCAAAT 3'

Fwd Tm 60.4°C

Rev Tm 60.6°C

GC 50%

Product 145 bp

Spans 2 introns

NCBI BLAST

NM_173354 · chr21

109 2 genes · 2 transcripts

FWD 5' TCGTGAGGTTCAGCTGATGA 3'

REV 5' GTGCCCGTTGGAAGTCAAAT 3'

Fwd Tm 60.4°C

Rev Tm 60.6°C

GC 50%

Product 145 bp

Spans 2 introns

NCBI BLAST