SMAD4 10 primer pairs found

Human

Show all (including non-specific)

NM_005359 · chr18

119 Specific

FWD 5' CCGGACATTACTGGCCTGTT 3'

REV 5' TAGGGCAGCTTGAAGGAACC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_005359 · chr18

117 Specific

FWD 5' CCGGACATTACTGGCCTGTT 3'

REV 5' GGGCAGCTTGAAGGAACCTT 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_005359 · chr18

116 Specific

FWD 5' CACAAGTCAGCCTGCCAGTA 3'

REV 5' CAGTCCAGGTGGTAGTGCTG 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_005359 · chr18

116 Specific

FWD 5' TGGCCTGTTCACAATGAGCT 3'

REV 5' TAGGGCAGCTTGAAGGAACC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 50%

Product 136 bp

Spans 2 introns

NM_005359 · chr18

116 Specific

FWD 5' ATCCCGGACATTACTGGCCT 3'

REV 5' GGGCAGCTTGAAGGAACCTT 3'

Fwd Tm 62.9°C

Rev Tm 62.5°C

GC 55%

Product 148 bp

Spans 2 introns

NM_005359 · chr18

116 Specific

FWD 5' CTGGCCTGTTCACAATGAGC 3'

REV 5' TAGGGCAGCTTGAAGGAACC 3'

Fwd Tm 61.2°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 2 introns

NM_005359 · chr18

116 Specific

FWD 5' CACAAGTCAGCCTGCCAGTA 3'

REV 5' AGTCCAGGTGGTAGTGCTGT 3'

Fwd Tm 61.8°C

Rev Tm 62.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_005359 · chr18

115 Specific

FWD 5' AACAGCACTACCACCTGGAC 3'

REV 5' AGGCTGGAATGCAAGCTCAT 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_005359 · chr18

115 Specific

FWD 5' CCATCCCGGACATTACTGGC 3'

REV 5' GGGCAGCTTGAAGGAACCTT 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 60%

Product 150 bp

Spans 2 introns

NM_005359 · chr18

115 Specific

FWD 5' CATCCCGGACATTACTGGCC 3'

REV 5' GGGCAGCTTGAAGGAACCTT 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 60%

Product 149 bp

Spans 2 introns