SMIM20 10 primer pairs found

Human

Show all (including non-specific)

NM_001145432 · chr4

114 Specific

FWD 5' GCCGCCTTCTATCCCATCTA 3'

REV 5' CCTGCCAAATGGATCAGACC 3'

Fwd Tm 60.7°C

Rev Tm 60.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001145432 · chr4

114 Specific

FWD 5' TATCCCATCTACTTCCGGCC 3'

REV 5' CCTGCCAAATGGATCAGACC 3'

Fwd Tm 60.5°C

Rev Tm 60.5°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001145432 · chr4

112 Specific

FWD 5' TATCCCATCTACTTCCGGCC 3'

REV 5' ACAGCCCTCTCATTTCCTGC 3'

Fwd Tm 60.5°C

Rev Tm 62.0°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001145432 · chr4

112 Specific

FWD 5' ATCCCATCTACTTCCGGCCC 3'

REV 5' AGCCCTCTCATTTCCTGCCA 3'

Fwd Tm 63.4°C

Rev Tm 63.4°C

GC 60%

Product 144 bp

Spans 2 introns

NM_001145432 · chr4

111 Specific

FWD 5' TATCCCATCTACTTCCGGCC 3'

REV 5' GACAGCCCTCTCATTTCCTG 3'

Fwd Tm 60.5°C

Rev Tm 59.9°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001145432 · chr4

111 Specific

FWD 5' TATCCCATCTACTTCCGGCC 3'

REV 5' GCCCTCTCATTTCCTGCCAA 3'

Fwd Tm 60.5°C

Rev Tm 62.3°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001145432 · chr4

111 Specific

FWD 5' TATCCCATCTACTTCCGGCC 3'

REV 5' TGACAGCCCTCTCATTTCCT 3'

Fwd Tm 60.5°C

Rev Tm 60.3°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001145432 · chr4

111 Specific

FWD 5' ATCCCATCTACTTCCGGCCC 3'

REV 5' GCCCTCTCATTTCCTGCCAA 3'

Fwd Tm 63.4°C

Rev Tm 62.3°C

GC 60%

Product 143 bp

Spans 2 introns

NM_001145432 · chr4

110 Specific

FWD 5' ATCCCATCTACTTCCGGCCC 3'

REV 5' ACAGCCCTCTCATTTCCTGC 3'

Fwd Tm 63.4°C

Rev Tm 62.0°C

GC 60%

Product 146 bp

Spans 2 introns

NM_001145432 · chr4

109 Specific

FWD 5' ATCCCATCTACTTCCGGCCC 3'

REV 5' CAGCCCTCTCATTTCCTGCC 3'

Fwd Tm 63.4°C

Rev Tm 62.8°C

GC 60%

Product 145 bp

Spans 2 introns