SRD5A1 30 primer pairs found

Human

Show all (including non-specific)

NM_001324323 · chr5

109 1 gene · 3 transcripts

FWD 5' TCATGAGTGGTACCTCCGGA 3'

REV 5' GAGAAGCGCCATTGGAAAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001324322 · chr5

109 1 gene · 3 transcripts

FWD 5' TCATGAGTGGTACCTCCGGA 3'

REV 5' GAGAAGCGCCATTGGAAAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001047 · chr5

109 1 gene · 3 transcripts

FWD 5' TCATGAGTGGTACCTCCGGA 3'

REV 5' GAGAAGCGCCATTGGAAAGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001324323 · chr5

108 Specific

FWD 5' CCACTACGGGCATCGGAAAT 3'

REV 5' TCAGATCCGGGCAGACAATG 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001324323 · chr5

108 1 gene · 2 transcripts

FWD 5' CTGTTGGCGTGTACAATGGC 3'

REV 5' CATGCCCGTTAACCACAAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001047 · chr5

108 1 gene · 2 transcripts

FWD 5' CTGTTGGCGTGTACAATGGC 3'

REV 5' CATGCCCGTTAACCACAAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' ATGACTTTACCCTGGCGCAC 3'

REV 5' ACACGCCAACAGTGGCATAG 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' GACTTTACCCTGGCGCACAT 3'

REV 5' ACACGCCAACAGTGGCATAG 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' GCCATGTTCCTCGTCCACTA 3'

REV 5' GATTCAGATCCGGGCAGACA 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 113 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' GCCATGTTCCTCGTCCACTA 3'

REV 5' TGATTCAGATCCGGGCAGAC 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' CCACTACGGGCATCGGAAAT 3'

REV 5' GATTCAGATCCGGGCAGACA 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' CCACTACGGGCATCGGAAAT 3'

REV 5' TGATTCAGATCCGGGCAGAC 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001324323 · chr5

107 Specific

FWD 5' ACTTTACCCTGGCGCACATG 3'

REV 5' ACACGCCAACAGTGGCATAG 3'

Fwd Tm 62.7°C

Rev Tm 62.6°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001047 · chr5

107 Specific

FWD 5' GCCATGTTCCTCGTCCACTA 3'

REV 5' GCCATTGTACACGCCAACAG 3'

Fwd Tm 61.3°C

Rev Tm 61.6°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001324322 · chr5

106 1 gene · 2 transcripts

FWD 5' GCCATGTTCCTCGTCCACTA 3'

REV 5' GCATAGCCACACCACTCCAT 3'

Fwd Tm 61.3°C

Rev Tm 62.0°C

GC 55%

Product 198 bp

Spans 2 introns

NM_001047 · chr5

106 Specific

FWD 5' ATCCTCCTGGCCATGTTCCT 3'

REV 5' GTACACGCCAACAGTGGCAT 3'

Fwd Tm 62.9°C

Rev Tm 62.9°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001047 · chr5

106 Specific

FWD 5' ATCCTCCTGGCCATGTTCCT 3'

REV 5' GCCAACAGTGGCATAGGCTT 3'

Fwd Tm 62.9°C

Rev Tm 62.8°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001047 · chr5

106 Specific

FWD 5' ATCCTCCTGGCCATGTTCCT 3'

REV 5' ACACGCCAACAGTGGCATAG 3'

Fwd Tm 62.9°C

Rev Tm 62.6°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001047 · chr5

106 1 gene · 2 transcripts

FWD 5' GCCACTGTTGGCGTGTACAA 3'

REV 5' ATGCCCGTTAACCACAAGCC 3'

Fwd Tm 63.0°C

Rev Tm 63.0°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001047 · chr5

106 Specific

FWD 5' ATCCTCCTGGCCATGTTCCT 3'

REV 5' TTGTACACGCCAACAGTGGC 3'

Fwd Tm 62.9°C

Rev Tm 63.0°C

GC 55%

Product 100 bp

Spans 1 intron

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