SSPN 20 primer pairs found

Human

Show all (including non-specific)

NM_005086 · chr12

120 Specific

FWD 5' GGCTGGGATCATTGTCTGCT 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001135823 · chr12

118 Specific

FWD 5' TCGGTCTGCTTAGTGGCCTA 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 117 bp

Spans 2 introns

NM_005086 · chr12

118 Specific

FWD 5' TGGGCTGGGATCATTGTCTG 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001135823 · chr12

117 Specific

FWD 5' GAGGCTCGGTCTGCTTAGTG 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 60%

Product 122 bp

Spans 2 introns

NM_001135823 · chr12

116 Specific

FWD 5' TTGGAGGCTCGGTCTGCTTA 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 62.9°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001135823 · chr12

115 Specific

FWD 5' CGGTCTGCTTAGTGGCCTAT 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 61.1°C

Rev Tm 62.1°C

GC 55%

Product 116 bp

Spans 2 introns

NM_001135823 · chr12

114 Specific

FWD 5' TTGGAGGCTCGGTCTGCTTA 3'

REV 5' CCAGGGCACTCAGCAGAAAG 3'

Fwd Tm 62.9°C

Rev Tm 62.9°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001135823 · chr12

113 Specific

FWD 5' TCGGTCTGCTTAGTGGCCTA 3'

REV 5' CCAGGGCACTCAGCAGAAAG 3'

Fwd Tm 62.3°C

Rev Tm 62.9°C

GC 55%

Product 118 bp

Spans 2 introns

NM_005086 · chr12

113 Specific

FWD 5' GGCTGGGATCATTGTCTGCT 3'

REV 5' CCAGGGCACTCAGCAGAAAG 3'

Fwd Tm 62.1°C

Rev Tm 62.9°C

GC 55%

Product 128 bp

Spans 2 introns

NM_005086 · chr12

112 Specific

FWD 5' TTGGGCTGGGATCATTGTCT 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 61.0°C

Rev Tm 62.1°C

GC 50%

Product 130 bp

Spans 2 introns

NM_005086 · chr12

112 Specific

FWD 5' GGGCTGGGATCATTGTCTGC 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 63.1°C

Rev Tm 62.1°C

GC 60%

Product 128 bp

Spans 2 introns

NM_005086 · chr12

112 Specific

FWD 5' TGGGCTGGGATCATTGTCTG 3'

REV 5' CCAGGGCACTCAGCAGAAAG 3'

Fwd Tm 61.8°C

Rev Tm 62.9°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001135823 · chr12

112 Specific

FWD 5' TTTGGAGGCTCGGTCTGCTT 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 63.7°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 2 introns

NM_005086 · chr12

112 Specific

FWD 5' GCTAGTCAGGGACACTCCAT 3'

REV 5' AGGGCACTCAGCAGAAAGTA 3'

Fwd Tm 60.6°C

Rev Tm 60.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001135823 · chr12

111 Specific

FWD 5' CGGTCTGCTTAGTGGCCTAT 3'

REV 5' AGGGCACTCAGCAGAAAGTA 3'

Fwd Tm 61.1°C

Rev Tm 60.5°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001135823 · chr12

111 Specific

FWD 5' TGGAGGCTCGGTCTGCTTAG 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 63.4°C

Rev Tm 62.1°C

GC 60%

Product 124 bp

Spans 2 introns

NM_001135823 · chr12

111 Specific

FWD 5' GGAGGCTCGGTCTGCTTAGT 3'

REV 5' CAGGGCACTCAGCAGAAAGT 3'

Fwd Tm 63.4°C

Rev Tm 62.1°C

GC 60%

Product 123 bp

Spans 2 introns

NM_005086 · chr12

110 Specific

FWD 5' TGGGCTGGGATCATTGTCTG 3'

REV 5' AGGGCACTCAGCAGAAAGTA 3'

Fwd Tm 61.8°C

Rev Tm 60.5°C

GC 55%

Product 128 bp

Spans 2 introns

NM_005086 · chr12

110 Specific

FWD 5' GGGCTGGGATCATTGTCTGC 3'

REV 5' CCAGGGCACTCAGCAGAAAG 3'

Fwd Tm 63.1°C

Rev Tm 62.9°C

GC 60%

Product 129 bp

Spans 2 introns

NM_005086 · chr12

110 Specific

FWD 5' GGCTGGGATCATTGTCTGCT 3'

REV 5' AGGGCACTCAGCAGAAAGTA 3'

Fwd Tm 62.1°C

Rev Tm 60.5°C

GC 55%

Product 126 bp

Spans 2 introns