Sema4f 30 primer pairs found

Human

Show all (including non-specific)

NM_001271661 · chr2

119 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' GGACAGAGCAGGTCAGCTTT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 137 bp

Spans 2 introns

NM_004263 · chr2

119 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' AAGGTGCCACAAGTGAGGAG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' CCGGCCACTCTCAAGTCTTT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 111 bp

Spans 2 introns

NM_004263 · chr2

119 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' GAAGGTGCCACAAGTGAGGA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' TTCAACCTGCTGGAACCTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 93 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' CCGGCCACTCTCAAGTCTTT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCTTTATCCCTGCCCTTCT 3'

REV 5' CCGGCCACTCTCAAGTCTTT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' TGCTGGAACCTGGACACATC 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCTTTATCCCTGCCCTTCT 3'

REV 5' TGCTGGAACCTGGACACATC 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' CTGCTGGAACCTGGACACAT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCTTTATCCCTGCCCTTCT 3'

REV 5' CTGCTGGAACCTGGACACAT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Spans 2 introns

NM_004263 · chr2

119 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' AGTGAGGAGGTGAGAGGCAT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' TCTTCGCTTTATCCCTGCCC 3'

REV 5' CCGGCCACTCTCAAGTCTTT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001271662 · chr2

119 1 gene · 2 transcripts

FWD 5' TCGCTTTATCCCTGCCCTTC 3'

REV 5' CCGGCCACTCTCAAGTCTTT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001271661 · chr2

119 Specific

FWD 5' TCGCTTTATCCCTGCCCTTC 3'

REV 5' AACGTCGTCCATCTCTGCTG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001271661 · chr2

119 Specific

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' AACGTCGTCCATCTCTGCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001271661 · chr2

119 Specific

FWD 5' CGCTTTATCCCTGCCCTTCT 3'

REV 5' AACGTCGTCCATCTCTGCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_004263 · chr2

119 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' GAGGTGAGAGGCATTGGCAA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001271661 · chr2

119 1 gene · 2 transcripts

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' AACGTCGTCCATCTCTGCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 113 bp

Spans 2 introns

NM_004263 · chr2

118 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' GGCAATGGCGAGAATCTGGA 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 105 bp

Spans 2 introns

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