Sf3a1 10 primer pairs found

Human

Show all (including non-specific)

NM_005877 · chr22

116 Specific

FWD 5' ACGGACCAGGTCTCTGTCAT 3'

REV 5' CGCCATTGGCCATGTTGTAG 3'

Fwd Tm 62.3°C

Rev Tm 61.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_005877 · chr22

116 Specific

FWD 5' CGGACCAGGTCTCTGTCATT 3'

REV 5' CGCCATTGGCCATGTTGTAG 3'

Fwd Tm 61.0°C

Rev Tm 61.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_005877 · chr22

114 Specific

FWD 5' ACAGCACAGCCTCTTCAACT 3'

REV 5' GCCCATTCCACTCGGTAACA 3'

Fwd Tm 61.3°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NM_005877 · chr22

112 Specific

FWD 5' ACGGACCAGGTCTCTGTCAT 3'

REV 5' GCGCCATTGGCCATGTTGTA 3'

Fwd Tm 62.3°C

Rev Tm 63.6°C

GC 55%

Product 137 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' CAGCCCACAGAAGAAGAAGC 3'

REV 5' GCTTCAAATTCAGGCCCGTT 3'

Fwd Tm 60.6°C

Rev Tm 61.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' ACAAGGGTCCAGTGTCCATC 3'

REV 5' GCATGCCTGTGGCTTCATGA 3'

Fwd Tm 61.4°C

Rev Tm 63.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' CAAGGGTCCAGTGTCCATCA 3'

REV 5' GCATGCCTGTGGCTTCATGA 3'

Fwd Tm 61.4°C

Rev Tm 63.1°C

GC 55%

Product 149 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' CACAGCACAGCCTCTTCAAC 3'

REV 5' CCCATTCCACTCGGTAACAC 3'

Fwd Tm 61.0°C

Rev Tm 60.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' CGGACCAGGTCTCTGTCATT 3'

REV 5' GCCATTGGCCATGTTGTAGT 3'

Fwd Tm 61.0°C

Rev Tm 60.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_005877 · chr22

111 Specific

FWD 5' AGCCCACAGAAGAAGAAGCA 3'

REV 5' GCTTCAAATTCAGGCCCGTT 3'

Fwd Tm 61.1°C

Rev Tm 61.0°C

GC 50%

Product 145 bp

Spans 2 introns