Spc25 20 primer pairs found

Human

Show all (including non-specific)

NM_020675 · chr2

108 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' ATCTGACAGGAGGTGTCCGT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 110 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

108 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' ATCTGACAGGAGGTGTCCGT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 110 bp

Spans 1 intron

NM_020675 · chr2

108 Specific

FWD 5' TAGAGTCCGGCTGTTGGCTA 3'

REV 5' TGCCAGTTCGTCCTCTACCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 111 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

108 Specific

FWD 5' TAGAGTCCGGCTGTTGGCTA 3'

REV 5' TGCCAGTTCGTCCTCTACCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 111 bp

Spans 1 intron

NM_020675 · chr2

107 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' TGACAGGAGGTGTCCGTACT 3'

Fwd Tm 62.4°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

107 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' TGACAGGAGGTGTCCGTACT 3'

Fwd Tm 62.4°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_020675 · chr2

106 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' TCTGACAGGAGGTGTCCGTA 3'

Fwd Tm 62.4°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

106 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' TCTGACAGGAGGTGTCCGTA 3'

Fwd Tm 62.4°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 1 intron

NM_020675 · chr2

106 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' GCCAGTTCGTCCTCTACCAT 3'

Fwd Tm 61.4°C

Rev Tm 61.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

106 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' GCCAGTTCGTCCTCTACCAT 3'

Fwd Tm 61.4°C

Rev Tm 61.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_020675 · chr2

106 Specific

FWD 5' TAGAGTCCGGCTGTTGGCTA 3'

REV 5' GTGCCAGTTCGTCCTCTACC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

106 Specific

FWD 5' TAGAGTCCGGCTGTTGGCTA 3'

REV 5' GTGCCAGTTCGTCCTCTACC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 1 intron

NM_020675 · chr2

105 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' GCCATCTGACAGGAGGTGTC 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 113 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

105 Specific

FWD 5' GCCTGCGAAGCATTGTCCTA 3'

REV 5' GCCATCTGACAGGAGGTGTC 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 113 bp

Spans 1 intron

NM_020675 · chr2

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' AGTGCCAGTTCGTCCTCTAC 3'

Fwd Tm 61.4°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' AGTGCCAGTTCGTCCTCTAC 3'

Fwd Tm 61.4°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 1 intron

NM_020675 · chr2

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' GTGCCAGTTCGTCCTCTACC 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' GTGCCAGTTCGTCCTCTACC 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_020675 · chr2

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' TGCCAGTTCGTCCTCTACCA 3'

Fwd Tm 61.4°C

Rev Tm 62.5°C

GC 55%

Product 148 bp

Spans 1 intron

NM_020675 · chr2_GL383522v1_alt

105 Specific

FWD 5' GAAATCGGAAAGTTGGCGGG 3'

REV 5' TGCCAGTTCGTCCTCTACCA 3'

Fwd Tm 61.4°C

Rev Tm 62.5°C

GC 55%

Product 148 bp

Spans 1 intron