TMEM258 10 primer pairs found

Human

Show all (including non-specific)

NM_014206 · chr11

111 Specific

FWD 5' AAATGGAGCTCGAGGCCATG 3'

REV 5' CTTGGTAGAGGTGACCTCGT 3'

Fwd Tm 62.3°C

Rev Tm 60.4°C

GC 55%

Product 137 bp

Spans 2 introns

NM_014206 · chr11

111 Specific

FWD 5' AAATGGAGCTCGAGGCCATG 3'

REV 5' ACTTGGTAGAGGTGACCTCG 3'

Fwd Tm 62.3°C

Rev Tm 60.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_014206 · chr11

110 Specific

FWD 5' GTTCTTCACCGCCTGGTTCT 3'

REV 5' AAGCCCATGAAGAGTGAGGC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 108 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' TGGCATCTACGTGTGAGCAC 3'

REV 5' TGGGACACTTCCAGCAACAG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' TGGGTTGGCATCTACGTGTG 3'

REV 5' GGGACACTTCCAGCAACAGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 103 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' TGGGTTGGCATCTACGTGTG 3'

REV 5' TGGGACACTTCCAGCAACAG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 104 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' TGGCATCTACGTGTGAGCAC 3'

REV 5' GGGACACTTCCAGCAACAGT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 98 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' GTTCTTCACCGCCTGGTTCT 3'

REV 5' CACACGTAGATGCCAACCCA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' GCCTCACTCTTCATGGGCTT 3'

REV 5' TGGGACACTTCCAGCAACAG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 1 intron

NM_014206 · chr11

110 Specific

FWD 5' GCCTCACTCTTCATGGGCTT 3'

REV 5' GGGACACTTCCAGCAACAGT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron