TOP3A 20 primer pairs found

Human

Show all (including non-specific)

NM_001320759 · chr17

119 1 gene · 2 transcripts

FWD 5' ATGAGGCGGAGAGAAGGACT 3'

REV 5' ACAAGAGGGTTGCAGCTCTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 90 bp

Spans 2 introns

NM_001320759 · chr17

119 1 gene · 2 transcripts

FWD 5' ATGAGGCGGAGAGAAGGACT 3'

REV 5' GACAAGAGGGTTGCAGCTCT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 91 bp

Spans 2 introns

NM_001320759 · chr17

118 Specific

FWD 5' TCATCTGTATGGCCAGGCAG 3'

REV 5' GGCGAGTCTCTCGTTCCAAA 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001320759 · chr17

118 Specific

FWD 5' CATCTGTATGGCCAGGCAGA 3'

REV 5' GGCGAGTCTCTCGTTCCAAA 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 116 bp

Spans 2 introns

NM_001320759 · chr17

118 1 gene · 2 transcripts

FWD 5' TGAGGCGGAGAGAAGGACTT 3'

REV 5' GACAAGAGGGTTGCAGCTCT 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 90 bp

Spans 2 introns

NM_001320759 · chr17

117 1 gene · 2 transcripts

FWD 5' TGTCAAACGGTCGCATGAGG 3'

REV 5' ACAAGAGGGTTGCAGCTCTG 3'

Fwd Tm 62.5°C

Rev Tm 62.1°C

GC 55%

Product 104 bp

Spans 2 introns

NM_001320759 · chr17

117 1 gene · 2 transcripts

FWD 5' GGCGGAGAGAAGGACTTTCA 3'

REV 5' AGAGGACAAGAGGGTTGCAG 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 91 bp

Spans 2 introns

NM_001320759 · chr17

117 1 gene · 2 transcripts

FWD 5' CATGAGGCGGAGAGAAGGAC 3'

REV 5' GACAAGAGGGTTGCAGCTCT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 60%

Product 92 bp

Spans 2 introns

NM_001320759 · chr17

117 Specific

FWD 5' TCATCTGTATGGCCAGGCAG 3'

REV 5' AGTCCAGATCACCAGAGCCT 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001320759 · chr17

117 Specific

FWD 5' CATCTGTATGGCCAGGCAGA 3'

REV 5' AGTCCAGATCACCAGAGCCT 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 142 bp

Spans 2 introns

NM_004618 · chr17

114 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' TGGCGAGTCTCTCGTTCCAA 3'

Fwd Tm 63.4°C

Rev Tm 63.0°C

GC 55%

Product 107 bp

Spans 2 introns

NM_004618 · chr17

113 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' AGTCCAGATCACCAGAGCCT 3'

Fwd Tm 63.4°C

Rev Tm 62.2°C

GC 55%

Product 132 bp

Spans 2 introns

NM_004618 · chr17

112 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' TCCAGATCACCAGAGCCTGG 3'

Fwd Tm 63.4°C

Rev Tm 63.3°C

GC 55%

Product 130 bp

Spans 2 introns

NM_004618 · chr17

112 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' GGCGAGTCTCTCGTTCCAAA 3'

Fwd Tm 63.4°C

Rev Tm 61.7°C

GC 55%

Product 106 bp

Spans 2 introns

NM_004618 · chr17

112 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' CTGGCGAGTCTCTCGTTCCA 3'

Fwd Tm 63.4°C

Rev Tm 63.5°C

GC 55%

Product 108 bp

Spans 2 introns

NM_004618 · chr17

112 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' ACTGGCGAGTCTCTCGTTCC 3'

Fwd Tm 63.4°C

Rev Tm 63.5°C

GC 55%

Product 109 bp

Spans 2 introns

NM_004618 · chr17

111 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' CACTGGCGAGTCTCTCGTTC 3'

Fwd Tm 63.4°C

Rev Tm 62.1°C

GC 55%

Product 110 bp

Spans 2 introns

NM_004618 · chr17

110 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' GTCCAGATCACCAGAGCCTG 3'

Fwd Tm 63.4°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 2 introns

NM_004618 · chr17

110 Specific

FWD 5' AAATGGCAGAGCTGCAACCC 3'

REV 5' CAGTCCAGATCACCAGAGCC 3'

Fwd Tm 63.4°C

Rev Tm 61.9°C

GC 55%

Product 133 bp

Spans 2 introns

NM_004618 · chr17

110 1 gene · 2 transcripts

FWD 5' AGACCATCAAAGCCCGGATG 3'

REV 5' ATCAGCTTCCAGTTCAGCCC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron