NM_000549 · chr1

110 Specific

FWD 5' CACCACCATCTGTGCTGGAT 3'

REV 5' AACATGGAGTGGGCATCCTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

110 Specific

FWD 5' CACCACCATCTGTGCTGGAT 3'

REV 5' CAACATGGAGTGGGCATCCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

108 Specific

FWD 5' AACACCACCATCTGTGCTGG 3'

REV 5' AACATGGAGTGGGCATCCTG 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

108 Specific

FWD 5' AACACCACCATCTGTGCTGG 3'

REV 5' CAACATGGAGTGGGCATCCT 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 148 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

108 Specific

FWD 5' GTCACCACAGCATCTGCTCA 3'

REV 5' TCGCTTGCCCACATGTAAGG 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 91 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

108 Specific

FWD 5' TCACCACAGCATCTGCTCAC 3'

REV 5' TCGCTTGCCCACATGTAAGG 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 90 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

108 Specific

FWD 5' AACACCACCATCTGTGCTGG 3'

REV 5' AGCAACATGGAGTGGGCATC 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 150 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

107 Specific

FWD 5' AACACCACCATCTGTGCTGG 3'

REV 5' TGGAGTGGGCATCCTGGTAT 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 143 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

107 Specific

FWD 5' AACACCACCATCTGTGCTGG 3'

REV 5' ATGGAGTGGGCATCCTGGTA 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 1 intron

NCBI BLAST

NM_000549 · chr1

107 Specific

FWD 5' GTCACCACAGCATCTGCTCA 3'

REV 5' AAGACATCGCTTGCCCACAT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_001277991 · chr1

76 Single Exon 1 gene · 2 transcripts

FWD 5' TGTCCCAGGATGTTTGCACA 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 50%

Product 125 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

75 Single Exon 1 gene · 2 transcripts

FWD 5' CTGTCCCAGGATGTTTGCAC 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 60.9°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

74 Single Exon 1 gene · 2 transcripts

FWD 5' TCTGTCCCAGGATGTTTGCA 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 61.1°C

Rev Tm 61.6°C

GC 50%

Product 127 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

72 Single Exon 1 gene · 2 transcripts

FWD 5' TGCTCTGTCCCAGGATGTTT 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 60.9°C

Rev Tm 61.6°C

GC 50%

Product 130 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

72 Single Exon 1 gene · 2 transcripts

FWD 5' GCTCTGTCCCAGGATGTTTG 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 60.4°C

Rev Tm 61.6°C

GC 55%

Product 129 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

72 Single Exon 1 gene · 2 transcripts

FWD 5' CTCTGTCCCAGGATGTTTGC 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 60.4°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

71 Single Exon 1 gene · 2 transcripts

FWD 5' ATGCTCTGTCCCAGGATGTT 3'

REV 5' GCACTTGCCACACTTACAGC 3'

Fwd Tm 60.7°C

Rev Tm 61.6°C

GC 50%

Product 131 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

70 Single Exon 1 gene · 2 transcripts

FWD 5' GCTCTGTCCCAGGATGTTTG 3'

REV 5' TGCACTTGCCACACTTACAG 3'

Fwd Tm 60.4°C

Rev Tm 60.2°C

GC 55%

Product 130 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

70 Single Exon 1 gene · 2 transcripts

FWD 5' CTCTGTCCCAGGATGTTTGC 3'

REV 5' TGCACTTGCCACACTTACAG 3'

Fwd Tm 60.4°C

Rev Tm 60.2°C

GC 55%

Product 129 bp

Single-exon gene (no intron)

NCBI BLAST

NM_001277991 · chr1

70 Single Exon 1 gene · 2 transcripts

FWD 5' CTGTCCCAGGATGTTTGCAC 3'

REV 5' TGCACTTGCCACACTTACAG 3'

Fwd Tm 60.9°C

Rev Tm 60.2°C

GC 55%

Product 127 bp

Single-exon gene (no intron)

NCBI BLAST

TSHB 20 primer pairs found