TWIST1 10 primer pairs found

Human

Show all (including non-specific)

NM_000474 · chr7

102 Specific

FWD 5' GCCGGAGACCTAGATGTCAT 3'

REV 5' CCACGCCCTGTTTCTTTGAA 3'

Fwd Tm 60.6°C

Rev Tm 60.5°C

GC 55%

Product 150 bp

Spans 1 intron

NM_000474 · chr7

97 Specific

FWD 5' TTCTCGGTCTGGAGGATGGA 3'

REV 5' CCAGCTCCAGAGTCTCTAGA 3'

Fwd Tm 61.8°C

Rev Tm 59.4°C

GC 55%

Product 148 bp

Spans 1 intron

NM_000474 · chr7

97 Specific

FWD 5' TTCTCGGTCTGGAGGATGGA 3'

REV 5' TCCAGCTCCAGAGTCTCTAG 3'

Fwd Tm 61.8°C

Rev Tm 59.4°C

GC 55%

Product 149 bp

Spans 1 intron

NM_000474 · chr7

95 Specific

FWD 5' CCTTCTCGGTCTGGAGGATG 3'

REV 5' CCAGCTCCAGAGTCTCTAGA 3'

Fwd Tm 61.3°C

Rev Tm 59.4°C

GC 60%

Product 150 bp

Spans 1 intron

NM_000474 · chr7

95 Specific

FWD 5' CTTCTCGGTCTGGAGGATGG 3'

REV 5' CCAGCTCCAGAGTCTCTAGA 3'

Fwd Tm 61.3°C

Rev Tm 59.4°C

GC 60%

Product 149 bp

Spans 1 intron

NM_000474 · chr7

95 Specific

FWD 5' CTTCTCGGTCTGGAGGATGG 3'

REV 5' TCCAGCTCCAGAGTCTCTAG 3'

Fwd Tm 61.3°C

Rev Tm 59.4°C

GC 60%

Product 150 bp

Spans 1 intron

NM_000474 · chr7

94 Specific

FWD 5' GGCCGGAGACCTAGATGTCA 3'

REV 5' CCCACGCCCTGTTTCTTTGA 3'

Fwd Tm 62.9°C

Rev Tm 62.6°C

GC 60%

Product 152 bp

Spans 1 intron

NM_000474 · chr7

93 Specific

FWD 5' GCCGGAGACCTAGATGTCAT 3'

REV 5' GGCCTGTCTCGCTTTCTCTT 3'

Fwd Tm 60.6°C

Rev Tm 61.8°C

GC 55%

Product 183 bp

Spans 1 intron

NM_000474 · chr7

93 Specific

FWD 5' TCTCGGTCTGGAGGATGGAG 3'

REV 5' CCAGCTCCAGAGTCTCTAGA 3'

Fwd Tm 62.4°C

Rev Tm 59.4°C

GC 60%

Product 147 bp

Spans 1 intron

NM_000474 · chr7

93 Specific

FWD 5' TCTCGGTCTGGAGGATGGAG 3'

REV 5' TCCAGCTCCAGAGTCTCTAG 3'

Fwd Tm 62.4°C

Rev Tm 59.4°C

GC 60%

Product 148 bp

Spans 1 intron