No gene-specific primers found for U2af1. Showing all 60 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

U2af1 60 primer pairs found

Human

Show all (including non-specific)

NM_001025204 · chr21

119 2 genes · 2 transcripts

FWD 5' GCTCACACTACCATTGCCCT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 102 bp

Spans 2 introns

NCBI BLAST

NM_001025204 · chr21

119 2 genes · 2 transcripts

FWD 5' GCTCACACTACCATTGCCCT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 102 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 110 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 110 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 110 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 110 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' TGCATCTCCACATCGCTCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' TGCATCTCCACATCGCTCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' TGCATCTCCACATCGCTCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' AACCGACGTTTAGCCAGACC 3'

REV 5' TGCATCTCCACATCGCTCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_001025204 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_001025204 · chr21

119 2 genes · 7 transcripts

FWD 5' ATGGGAGAATGCACACGAGG 3'

REV 5' TCTAGACCGAGAACGACGCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' CCGACGTTTAGCCAGACCAT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' CCGACGTTTAGCCAGACCAT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 2 introns

NCBI BLAST

NM_006758 · chr21

119 2 genes · 6 transcripts

FWD 5' CCGACGTTTAGCCAGACCAT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 2 introns

NCBI BLAST

NM_001025203 · chr21

119 2 genes · 6 transcripts

FWD 5' CCGACGTTTAGCCAGACCAT 3'

REV 5' CTGCATCTCCACATCGCTCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 2 introns

NCBI BLAST

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