a2ml 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001139479 · chr15

118 Specific

FWD 5' TCAGCCATCCGAGAGAAAGC 3'

REV 5' ACCTCAGGACAAAGGCAGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' TCAGCCATCCGAGAGAAAGC 3'

REV 5' GACCTCAGGACAAAGGCAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' ATCCGAGAGAAAGCCAGCAG 3'

REV 5' ACCTCAGGACAAAGGCAGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' ATCCGAGAGAAAGCCAGCAG 3'

REV 5' GACCTCAGGACAAAGGCAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' TCAGCCATCCGAGAGAAAGC 3'

REV 5' CCTCAGGACAAAGGCAGTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' ATCCGAGAGAAAGCCAGCAG 3'

REV 5' CCTCAGGACAAAGGCAGTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' CATCCGAGAGAAAGCCAGCA 3'

REV 5' ACCTCAGGACAAAGGCAGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' CATCCGAGAGAAAGCCAGCA 3'

REV 5' GACCTCAGGACAAAGGCAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' CATCCGAGAGAAAGCCAGCA 3'

REV 5' CCTCAGGACAAAGGCAGTCA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001139479 · chr15

118 Specific

FWD 5' GACAGAGACCAACAGCTGGT 3'

REV 5' AGATTCTGCAGTGCTCGACC 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns