aaas 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_213225 · chr9

120 Specific

FWD 5' TCCTCCTTCCATGGCTCTCT 3'

REV 5' ATCCGTGTGAGGATGCCAAG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 2 introns

NM_213225 · chr9

120 Specific

FWD 5' CAGATGAAGTGCCCAGGTGT 3'

REV 5' GCCACCATCTCATCACTGCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 124 bp

Spans 2 introns

NM_213225 · chr9

120 Specific

FWD 5' AGATGAAGTGCCCAGGTGTG 3'

REV 5' GCCACCATCTCATCACTGCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 123 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' TACGCGCTCTCCTTCTCTGA 3'

REV 5' ACCTCGAGGATCCCAAGACA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' TCTACGCGCTCTCCTTCTCT 3'

REV 5' ACCTCGAGGATCCCAAGACA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 146 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' TCTACGCGCTCTCCTTCTCT 3'

REV 5' TCACCTCGAGGATCCCAAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 148 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' GATGAAGTGCCCAGGTGTGT 3'

REV 5' GCCACCATCTCATCACTGCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 122 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' AGTGCAGATGAAGTGCCCAG 3'

REV 5' GCCACCATCTCATCACTGCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 128 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' TGATCTCACAGGAGGTCCGA 3'

REV 5' TCACCTCGAGGATCCCAAGA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 129 bp

Spans 2 introns

NM_213225 · chr9

119 Specific

FWD 5' CGAGGTGAAAGACTCGCTGT 3'

REV 5' CAGCTTCGCCCATCACAAAC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 136 bp

Spans 2 introns