aagab 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001002423 · chr18

117 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' CCTGGTCTCCACCAATAGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

117 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' CCTGGTCTCCACCAATAGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001002423 · chr18

116 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' CTGGTCTCCACCAATAGCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.2°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

116 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' CTGGTCTCCACCAATAGCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.2°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001002423 · chr18

116 Specific

FWD 5' CGACAGCGATGAGGGTGTTA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.5°C

Rev Tm 61.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

116 Specific

FWD 5' CGACAGCGATGAGGGTGTTA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.5°C

Rev Tm 61.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001002423 · chr18

116 Specific

FWD 5' GCGATGAGGGTGTTAGCAGA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.0°C

GC 55%

Product 122 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

116 Specific

FWD 5' GCGATGAGGGTGTTAGCAGA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.0°C

GC 55%

Product 122 bp

Spans 2 introns

NM_001002423 · chr18

116 Specific

FWD 5' AGCGATGAGGGTGTTAGCAG 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.0°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

116 Specific

FWD 5' AGCGATGAGGGTGTTAGCAG 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.6°C

Rev Tm 61.0°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001002423 · chr18

115 Specific

FWD 5' CAGCGATGAGGGTGTTAGCA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

115 Specific

FWD 5' CAGCGATGAGGGTGTTAGCA 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001002423 · chr18

115 Specific

FWD 5' ACAGCGATGAGGGTGTTAGC 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

115 Specific

FWD 5' ACAGCGATGAGGGTGTTAGC 3'

REV 5' CCCAGTGGACTCTGGAAAGT 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001002423 · chr18

115 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' TCCTGGTCTCCACCAATAGC 3'

Fwd Tm 61.9°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

115 Specific

FWD 5' TGGAGACCCAGATGTGGAGA 3'

REV 5' TCCTGGTCTCCACCAATAGC 3'

Fwd Tm 61.9°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001002423 · chr18

114 Specific

FWD 5' CTGGAGACCCAGATGTGGAG 3'

REV 5' CTGGTCTCCACCAATAGCCA 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

114 Specific

FWD 5' CTGGAGACCCAGATGTGGAG 3'

REV 5' CTGGTCTCCACCAATAGCCA 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001002423 · chr18

114 Specific

FWD 5' GGAGACCCAGATGTGGAGAA 3'

REV 5' TCCTGGTCTCCACCAATAGC 3'

Fwd Tm 60.5°C

Rev Tm 60.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001002423 · chr18_KZ115558v1_alt

114 Specific

FWD 5' GGAGACCCAGATGTGGAGAA 3'

REV 5' TCCTGGTCTCCACCAATAGC 3'

Fwd Tm 60.5°C

Rev Tm 60.9°C

GC 55%

Product 149 bp

Spans 2 introns