aass 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001173985 · chr25

120 Specific

FWD 5' TTTGGTGACCGACTGTTCCC 3'

REV 5' GGTGTCAACTTCCCGTTGGA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001173985 · chr25

120 Specific

FWD 5' TTTGGTGACCGACTGTTCCC 3'

REV 5' AGGTGTCAACTTCCCGTTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' ATGCTCCCATCAGATGCCAC 3'

REV 5' AGGTGTCAACTTCCCGTTGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 96 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' CAGCATGCTGCCATACTCCT 3'

REV 5' ATGGTGATGCCAGCATCCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' CAGCATGCTGCCATACTCCT 3'

REV 5' GATGGTGATGCCAGCATCCT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' ATGCTCCCATCAGATGCCAC 3'

REV 5' GGTGTCAACTTCCCGTTGGA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' TCAGCATGCTGCCATACTCC 3'

REV 5' ATGGTGATGCCAGCATCCTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' TCAGCATGCTGCCATACTCC 3'

REV 5' GATGGTGATGCCAGCATCCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' GATGCTCCCATCAGATGCCA 3'

REV 5' GGTGTCAACTTCCCGTTGGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 2 introns

NM_001173985 · chr25

119 Specific

FWD 5' GATGCTCCCATCAGATGCCA 3'

REV 5' AGGTGTCAACTTCCCGTTGG 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 97 bp

Spans 2 introns