abi3bpb 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_199859 · chr22_KZ115652v1_alt

120 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 2 introns

NM_199859 · chr22

120 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

119 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 131 bp

Spans 2 introns

NM_199859 · chr22

119 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 131 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

119 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' CTCTTGGAGCAGCCACAGTT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 2 introns

NM_199859 · chr22

119 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' CTCTTGGAGCAGCCACAGTT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

119 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 2 introns

NM_199859 · chr22

119 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

119 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 2 introns

NM_199859 · chr22

119 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

118 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' CTCTTGGAGCAGCCACAGTT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_199859 · chr22

118 Specific

FWD 5' CCACTTCGACCTGTTCCCAA 3'

REV 5' CTCTTGGAGCAGCCACAGTT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

118 Specific

FWD 5' TTCCCACTTCGACCTGTTCC 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 2 introns

NM_199859 · chr22

118 Specific

FWD 5' TTCCCACTTCGACCTGTTCC 3'

REV 5' TGGCTCATTGGCTCTTGGAG 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

117 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TCATTGGCTCTTGGAGCAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.5°C

GC 55%

Product 139 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

117 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' GCTCATTGGCTCTTGGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_199859 · chr22

117 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' TCATTGGCTCTTGGAGCAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.5°C

GC 55%

Product 139 bp

Spans 2 introns

NM_199859 · chr22

117 Specific

FWD 5' CGTTCCCACTTCGACCTGTT 3'

REV 5' GCTCATTGGCTCTTGGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_199859 · chr22_KZ115652v1_alt

117 Specific

FWD 5' TTCCCACTTCGACCTGTTCC 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 61.5°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_199859 · chr22

117 Specific

FWD 5' TTCCCACTTCGACCTGTTCC 3'

REV 5' TCTTGGAGCAGCCACAGTTC 3'

Fwd Tm 61.5°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns