ablim3 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001080598 · chr14

119 Specific

FWD 5' GCCCGGTGTCAGATGATGTT 3'

REV 5' ATGGAGGCTTCTGATGTGCG 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

119 Specific

FWD 5' GCCCGGTGTCAGATGATGTT 3'

REV 5' ATGGAGGCTTCTGATGTGCG 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001080598 · chr14

119 Specific

FWD 5' GCCCGGTGTCAGATGATGTT 3'

REV 5' TGGAGGAGAGATGGAGGCTT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

119 Specific

FWD 5' GCCCGGTGTCAGATGATGTT 3'

REV 5' TGGAGGAGAGATGGAGGCTT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001080598 · chr14

119 Specific

FWD 5' ATGACAGCGGCAGTGAAGTC 3'

REV 5' AAGATGCCGCTCCAATCTGG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

119 Specific

FWD 5' ATGACAGCGGCAGTGAAGTC 3'

REV 5' AAGATGCCGCTCCAATCTGG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001080598 · chr14

119 Specific

FWD 5' ATGACAGCGGCAGTGAAGTC 3'

REV 5' AATCTGGCCCGGTCAACATC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 121 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

119 Specific

FWD 5' ATGACAGCGGCAGTGAAGTC 3'

REV 5' AATCTGGCCCGGTCAACATC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 121 bp

Spans 2 introns

NM_001080598 · chr14

119 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' CTTTGTGGCCTGTGCAATCC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

119 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' CTTTGTGGCCTGTGCAATCC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001080598 · chr14

118 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' ATCCAGGCAAAGAGGCTGAC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 92 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

118 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' ATCCAGGCAAAGAGGCTGAC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 92 bp

Spans 2 introns

NM_001080598 · chr14

118 Specific

FWD 5' TGCTCCGATGACAGGCTAGA 3'

REV 5' TGTCTGCTGTAGGTGGGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

118 Specific

FWD 5' TGCTCCGATGACAGGCTAGA 3'

REV 5' TGTCTGCTGTAGGTGGGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001080598 · chr14

118 Specific

FWD 5' GGCTCTCCTCGGTCAAACTT 3'

REV 5' GTGGCCTGTGCAATCCATTC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 94 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

118 Specific

FWD 5' GGCTCTCCTCGGTCAAACTT 3'

REV 5' GTGGCCTGTGCAATCCATTC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 94 bp

Spans 2 introns

NM_001080598 · chr14

118 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' GTGGCCTGTGCAATCCATTC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 119 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

118 Specific

FWD 5' CATGGGCCACAACAACACAG 3'

REV 5' GTGGCCTGTGCAATCCATTC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 119 bp

Spans 2 introns

NM_001080598 · chr14

118 Specific

FWD 5' GGCTCTCCTCGGTCAAACTT 3'

REV 5' CTTTGTGGCCTGTGCAATCC 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 98 bp

Spans 2 introns

NM_001080598 · chr14_KZ115456v1_alt

118 Specific

FWD 5' GGCTCTCCTCGGTCAAACTT 3'

REV 5' CTTTGTGGCCTGTGCAATCC 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 98 bp

Spans 2 introns