abr 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001368694 · chr5

120 Specific

FWD 5' GGCAAGGGTCAAGTACAGCT 3'

REV 5' TGTTTCTTAGACGGCGTCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001368694 · chr5

119 Specific

FWD 5' GTGGAGAAATGCAGCCAAGC 3'

REV 5' GCTTCGAGTAACCCGGTCAA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001368694 · chr5

119 Specific

FWD 5' GCAGCCAAGCCAACATTCAG 3'

REV 5' GCTTCGAGTAACCCGGTCAA 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001368694 · chr5

118 Specific

FWD 5' AGTACAGCTGGACCCACAGA 3'

REV 5' TGTTTCTTAGACGGCGTCCG 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001368694 · chr5

118 Specific

FWD 5' AGTACAGCTGGACCCACAGA 3'

REV 5' CGTCCGCTTCAAGCTCAAGT 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001368694 · chr5

118 Specific

FWD 5' CAAGTACAGCTGGACCCACA 3'

REV 5' TGTTTCTTAGACGGCGTCCG 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001368694 · chr5

117 Specific

FWD 5' CCCGTCACCAGCAACAAAGA 3'

REV 5' AAGAGTCCACCTCCAGCGTA 3'

Fwd Tm 62.6°C

Rev Tm 62.3°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001368694 · chr5

117 Specific

FWD 5' GGCAAGAGCTACCTGTTCCT 3'

REV 5' CTCAACACACATGCCTGCAG 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 104 bp

Spans 2 introns

NM_001368694 · chr5

117 Specific

FWD 5' TCCAAGGACTGCACTGCAAC 3'

REV 5' TCTCAGAGCGTCCTGCAGTA 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001368694 · chr5

117 Specific

FWD 5' GAAAGCGCAGAAGGGTCAGA 3'

REV 5' CTGAGGACAGCAGGAACAGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001100016 · chr5

116 1 gene · 2 transcripts

FWD 5' GCCTTCATGGAGGGCATTTC 3'

REV 5' TTGATCGGCTCCTTCTCTGC 3'

Fwd Tm 61.1°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001100016 · chr5

114 1 gene · 2 transcripts

FWD 5' GGCCTTCATGGAGGGCATTT 3'

REV 5' TTGATCGGCTCCTTCTCTGC 3'

Fwd Tm 62.7°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' CTGCCAGACCCAAACCTCAT 3'

REV 5' AGTCGGACCAAACACTGTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' GCCTTCATGGAGGGCATTTC 3'

REV 5' GTTGATCGGCTCCTTCTCTG 3'

Fwd Tm 61.1°C

Rev Tm 59.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' TGCCAGACCCAAACCTCATC 3'

REV 5' AGTCGGACCAAACACTGTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' GCCAGACCCAAACCTCATCA 3'

REV 5' AGTCGGACCAAACACTGTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' CCACAGTGTTTGGTCCGACT 3'

REV 5' GGCGGATGCTGCAGGTAATA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' GGCCTTCATGGAGGGCATTT 3'

REV 5' GATCGGCTCCTTCTCTGCCA 3'

Fwd Tm 62.7°C

Rev Tm 63.7°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' GGCCTTCATGGAGGGCATTT 3'

REV 5' TGATCGGCTCCTTCTCTGCC 3'

Fwd Tm 62.7°C

Rev Tm 63.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001100016 · chr5

110 1 gene · 2 transcripts

FWD 5' CCACAGTGTTTGGTCCGACT 3'

REV 5' AAGGATATGGGCGGATGCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 148 bp

Spans 1 intron