acaca 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001271308 · chr5_KZ115151v1_alt

120 Specific

FWD 5' TCTCTGCTGTTTGACCACGG 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001271308 · chr5

120 Specific

FWD 5' TCTCTGCTGTTTGACCACGG 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

119 Specific

FWD 5' TACCCTGGAGTGGAACAGGA 3'

REV 5' AGGGAAGCCCACTTGTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001271308 · chr5

119 Specific

FWD 5' TACCCTGGAGTGGAACAGGA 3'

REV 5' AGGGAAGCCCACTTGTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

119 Specific

FWD 5' CAGTTTCCCAGCCAGCAGAT 3'

REV 5' ATGTCCTCTGATGCCACTGC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001271308 · chr5

119 Specific

FWD 5' CAGTTTCCCAGCCAGCAGAT 3'

REV 5' ATGTCCTCTGATGCCACTGC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Predicted

FWD 5' CTGGACTGAAGGCTGCTGAA 3'

REV 5' GGAACCTGGAACCTCAGCTT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Specific

FWD 5' TACCCTGGAGTGGAACAGGA 3'

REV 5' CAGGGAAGCCCACTTGTTCA 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' TACCCTGGAGTGGAACAGGA 3'

REV 5' CAGGGAAGCCCACTTGTTCA 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Specific

FWD 5' TCCTGTTCAAGCTGTGTGGG 3'

REV 5' AGCCCACATAGCCTGACTTG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 118 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' TCCTGTTCAAGCTGTGTGGG 3'

REV 5' AGCCCACATAGCCTGACTTG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 118 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Specific

FWD 5' AGGTGGACCAGAGGTTCCAT 3'

REV 5' TTGAGCTCCAGCTGGAAAGC 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' AGGTGGACCAGAGGTTCCAT 3'

REV 5' TTGAGCTCCAGCTGGAAAGC 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Specific

FWD 5' CCACGGCATCCGAAGACTTA 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Specific

FWD 5' ACGGCATCCGAAGACTTACC 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' CCACGGCATCCGAAGACTTA 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' ACGGCATCCGAAGACTTACC 3'

REV 5' TCAAACTTGTCTCGAGCCCG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001271308 · chr5_KZ115151v1_alt

118 Predicted

FWD 5' TGGACTGAAGGCTGCTGAAC 3'

REV 5' GGAACCTGGAACCTCAGCTT 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' AGGTGGACCAGAGGTTCCAT 3'

REV 5' GGTTGAGCTCCAGCTGGAAA 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001271308 · chr5

118 Specific

FWD 5' TCCTGTTCAAGCTGTGTGGG 3'

REV 5' AGAGCCCACATAGCCTGACT 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 120 bp

Spans 2 introns